Sciatic nerve regeneration induced by glycosaminoglycan and laminin mimetic peptide nanofiber gels

Abstract

In the USA, 20 million patients suffer from neuropathy caused by peripheral nerve injuries, which costs approximately 150 billion annually. For longer nerve gaps and multiple injury sites, it is essential to use nerve guidance conduits for healthy pathfinding of regenerating axons. Here, extracellular matrix mimetic peptide nanofiber hydrogels were used for functionalizing guidance conduits to enhance neuronal regeneration in the distal stump of full transaction sciatic nerve injury in rats with functional repair. Conduits filled with heparan sulfate and laminin mimetic peptide nanofibers significantly improved electromyography response and promoted neuronal regeneration in a rat model of sciatic nerve defect. In addition, Schwann cells cultured on these nanofibers showed increased viability and significantly enhanced nerve growth factor (NGF) release. Overall, these results suggest that extracellular matrix mimetic peptide nanofibers present a promising treatment option for peripheral nerve injuries.