One-Step Fabrication of Biocompatible Multifaceted Nanocomposite Gels and Nanolayers

Date
2017
Authors
Topuz, F.
Bartneck, M.
Pan, Y.
Tacke, F.
Advisor
Supervisor
Co-Advisor
Co-Supervisor
Instructor
Source Title
Biomacromolecules
Print ISSN
1525-7797
Electronic ISSN
1526-4602
Publisher
American Chemical Society
Volume
18
Issue
2
Pages
386 - 397
Language
English
Type
Article
Journal Title
Journal ISSN
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Abstract

Nanocomposite gels are a fascinating class of polymeric materials with an integrative assembly of organic molecules and organic/inorganic nanoparticles, offering a unique hybrid network with synergistic properties. The mechanical properties of such networks are similar to those of natural tissues, which make them ideal biomaterial candidates for tissue engineering applications. Existing nanocomposite gel systems, however, lack many desirable gel properties, and their suitability for surface coatings is often limited. To address this issue, this article aims at generating multifunctional nanocomposite gels that are injectable with an appropriate time window, functional with bicyclononynes (BCN), biocompatible and slowly degradable, and possess high mechanical strength. Further, the in situ network-forming property of the proposed system allows the fabrication of ultrathin nanocomposite coatings in the submicrometer range with tunable wettability and roughness. Multifunctional nanocomposite gels were fabricated under cytocompatible conditions (pH 7.4 and T = 37 °C) using laponite clays, isocyanate (NCO)-terminated sP(EO-stat-PO) macromers, and clickable BCN. Several characterization techniques were employed to elucidate the structure-property relationships of the gels. Even though the NCO-sP(EO-stat-PO) macromers could form a hydrogel network in situ on contact with water, the incorporation of laponite led to significant improvement of the mechanical properties. BCN motifs with carbamate links were used for a metal-free click ligation with azide-functional molecules, and the subsequent gradual release of the tethered molecules through the hydrolysis of carbamate bonds was shown. The biocompatibility of the hydrogels was examined through murine macrophages, showing that the material composition strongly affects cell behavior.

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Keywords
Biocompatibility, Biomechanics, Characterization, Coatings, Fabrication, Gels, Hybrid materials, Mechanical properties, Molecules, Tissue, Tissue engineering, Characterization techniques, High mechanical strength, Material compositions, Multifunctional nanocomposites, Nano-composite coating, Structure property relationships, Synergistic properties, Tissue engineering applications, Animals, Biocompatible materials, Cell adhesion, Cells cultured, Hydrogels, Macrophages, Mice, Nanocomposites, Polymers
Citation
Published Version (Please cite this version)