Genetically programmed engineered cells for biomaterials synthesis

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buir.advisorŞeker, Urartu Özgür Şafak
dc.contributor.authorKırpat, Büşra Merve
dc.date.accessioned2021-01-18T11:32:33Z
dc.date.available2021-01-18T11:32:33Z
dc.date.copyright2021-01
dc.date.issued2021-01
dc.date.submitted2021-01-15
dc.descriptionCataloged from PDF version of article.en_US
dc.descriptionThesis (M.S.): Bilkent University, Department of Materials Science and Nanotechnology, İhsan Doğramacı Bilkent University, 2021.en_US
dc.descriptionIncludes bibliographical references (leaves 82-86).en_US
dc.description.abstractSeveral organisms can process nanomaterials and producing in various sizes and morphologies in mild conditions by utilizing specific proteins. In sea sponges, silicatein proteins play a key role in synthesizing silica nanoparticles the precursor silicic acid. Silaffin proteins in diatoms can also biomineralize silica. One subunit of silaffin called R5 peptide has a key role for nucleation and initiation of the nanoparticle formation and it has been shown that bacteria synthesized R5 peptide has ability to precipitate silica structures. These silica nanostructures can be utilized in many areas. Silica-based cements take attentions to make them useful in restorative dentistry and endodontics. In this work, a synthetic cell system has reprogrammed autotransporter (Ag43) system to display R5 peptide fused with fluorescent proteins. After displaying the fused proteins on the surface of bacteria or secreting them into environment, whole cell or the proteins are used to precipitate silica in the presence of precursor such as tetramethyl orthosilicate (TMOS). These silica structures are used to evaluate their in vitro effects on the proliferation of dental pulp stem cells (DPSCs) and their osteogenesis.en_US
dc.description.provenanceSubmitted by Betül Özen (ozen@bilkent.edu.tr) on 2021-01-18T11:32:33Z No. of bitstreams: 1 Busra_Merve_Kirpat_Thesis.pdf: 11958180 bytes, checksum: dd3dc113f9dfeccc4ff36538607759de (MD5)en
dc.description.provenanceMade available in DSpace on 2021-01-18T11:32:33Z (GMT). No. of bitstreams: 1 Busra_Merve_Kirpat_Thesis.pdf: 11958180 bytes, checksum: dd3dc113f9dfeccc4ff36538607759de (MD5) Previous issue date: 2021-01en
dc.description.statementofresponsibilityby Büşra Merve Kırpaten_US
dc.format.extentxviii, 117 leaves : color illustrations ; 30 cm.en_US
dc.identifier.itemidB124897
dc.identifier.urihttp://hdl.handle.net/11693/54893
dc.language.isoEnglishen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiomineralizationen_US
dc.subjectSilicaen_US
dc.subjectR5 peptideen_US
dc.subjectAg43en_US
dc.subjectDPSCen_US
dc.titleGenetically programmed engineered cells for biomaterials synthesisen_US
dc.title.alternativeBiyomateryal sentezi için genetiği değiştirilerek programlanan hücreleren_US
dc.typeThesisen_US
thesis.degree.disciplineMaterials Science and Nanotechnology
thesis.degree.grantorBilkent University
thesis.degree.levelMaster's
thesis.degree.nameMS (Master of Science)

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