Genetically programmed engineered cells for biomaterials synthesis

Date

2021-01

Editor(s)

Advisor

Şeker, Urartu Özgür Şafak

Supervisor

Co-Advisor

Co-Supervisor

Instructor

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Abstract

Several organisms can process nanomaterials and producing in various sizes and morphologies in mild conditions by utilizing specific proteins. In sea sponges, silicatein proteins play a key role in synthesizing silica nanoparticles the precursor silicic acid. Silaffin proteins in diatoms can also biomineralize silica. One subunit of silaffin called R5 peptide has a key role for nucleation and initiation of the nanoparticle formation and it has been shown that bacteria synthesized R5 peptide has ability to precipitate silica structures. These silica nanostructures can be utilized in many areas. Silica-based cements take attentions to make them useful in restorative dentistry and endodontics. In this work, a synthetic cell system has reprogrammed autotransporter (Ag43) system to display R5 peptide fused with fluorescent proteins. After displaying the fused proteins on the surface of bacteria or secreting them into environment, whole cell or the proteins are used to precipitate silica in the presence of precursor such as tetramethyl orthosilicate (TMOS). These silica structures are used to evaluate their in vitro effects on the proliferation of dental pulp stem cells (DPSCs) and their osteogenesis.

Source Title

Publisher

Course

Other identifiers

Book Title

Degree Discipline

Materials Science and Nanotechnology

Degree Level

Master's

Degree Name

MS (Master of Science)

Citation

Published Version (Please cite this version)

Language

English

Type