Production and structural characterization of biosurfactant produced by newly isolated staphylococcus xylosus STF1 from petroleum contaminated soil

Date
2015
Authors
Keskin, N. O. S.
Han, D.
Ozkan A.D.
Angun, P.
Umu, O. C. O.
Tekinay, T.
Advisor
Supervisor
Co-Advisor
Co-Supervisor
Instructor
Source Title
Journal of Petroleum Science and Engineering
Print ISSN
0920-4105
Electronic ISSN
Publisher
Elsevier BV
Volume
133
Issue
Pages
689 - 694
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Series
Abstract

Petroleum-contaminated soil was used to isolate and characterize biosurfactant producing bacteria. The strain could produce higher amount of biosurfactant in medium supplemented with motor oil as sole source of carbon and energy. A new biosurfactant producing bacterium, designated as Staphylococcus xylosus STF1 based on morphological, physiological, biochemical tests and 16S rRNA gene sequencing. The isolated bacterium was first screened for the ability to produce biosurfactant. Partial sequence of STF1 strain of 16S rDNA gene was highly similar to those of various members of the family Staphylococcaceae. Biochemical characterizations including FT-IR, Raman spectroscopy and Mass spectroscopy studies suggested the biosurfactant to be lipopeptide. Study also confirmed that the cell free supernatant exhibited high emulsifying activity against the different hydrocarbons. Moreover, the partially purified biosurfactant exhibited antimicrobial activity by inhibiting the growth of several bacterial species. The strain could be a potential candidate for the production of polypeptide biosurfactant which could be useful in a variety of biotechnological and industrial processes, particularly in the food and oil industry. © 2015 Elsevier B.V.

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Book Title
Keywords
Biosurfactant, FT-IR, Lipopeptide, Raman spectroscopy, Staphylococcus, Bacteria, Carbon, Emulsification, Genes, Mass spectrometry, Oils and fats, Raman spectroscopy, RNA, Soil pollution, Surface active agents, Anti-microbial activity, Bio surfactant, Biochemical characterization, Lipopeptides, Petroleum-contaminated soil, Staphylococcus, Structural characterization, Biomolecules, Bacterium, DNA, FTIR spectroscopy, Mass spectrometry, Microbial activity, Peptide, Petroleum, Surfactant, Bacteria (microorganisms), Staphylococcaceae, Staphylococcus, Staphylococcus xylosus
Citation
Published Version (Please cite this version)