Inhibition of VEGF mediated corneal neovascularization by anti-angiogenic peptide nanofibers

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buir.contributor.authorGüler, Mustafa O.
dc.citation.epage132en_US
dc.citation.spage124en_US
dc.citation.volumeNumber107en_US
dc.contributor.authorSenturk, B.en_US
dc.contributor.authorCubuk, M. O.en_US
dc.contributor.authorOzmen, M. C.en_US
dc.contributor.authorAydin B.en_US
dc.contributor.authorGüler, Mustafa O.en_US
dc.contributor.authorTekinay, A. B.en_US
dc.date.accessioned2018-04-12T10:56:03Z
dc.date.available2018-04-12T10:56:03Z
dc.date.issued2016-11en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.description.abstractAtypical angiogenesis is one of the major symptoms of severe eye diseases, including corneal neovascularization, and the complex nature of abnormal vascularization requires targeted methods with high biocompatibility. The targeting of VEGF is the most common approach for preventing angiogenesis, and the LPPR peptide sequence is known to strongly inhibit VEGF activity by binding to the VEGF receptor neuropilin-1. Here, the LPPR epitope is presented on a peptide amphiphile nanofiber system to benefit from multivalency and increase the anti-angiogenic function of the epitope. Peptide amphiphile nanofibers are especially useful for ocular delivery applications due to their ability to remain on the site of interest for extended periods of time, facilitating the long-term presentation of bioactive sequences. Consequently, the LPPR sequence was integrated into a self-assembled peptide amphiphile network to increase its efficiency in the prevention of neovascularization. Anti-angiogenic effects of the peptide nanofibers were investigated by using both in vitro and in vivo models. LPPR-PA nanofibers inhibited endothelial cell proliferation, tube formation, and migration to a greater extent than the soluble LPPR peptide in vitro. In addition, the LPPR-PA nanofiber system led to the prevention of vascular maturation and the regression of angiogenesis in a suture-induced corneal angiogenesis model. These results show that the anti-angiogenic activity exhibited by LPPR peptide nanofibers may be utilized as a promising approach for the treatment of corneal angiogenesis.en_US
dc.description.provenanceMade available in DSpace on 2018-04-12T10:56:03Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 179475 bytes, checksum: ea0bedeb05ac9ccfb983c327e155f0c2 (MD5) Previous issue date: 2016en
dc.identifier.doi10.1016/j.biomaterials.2016.08.045en_US
dc.identifier.issn0142-9612
dc.identifier.urihttp://hdl.handle.net/11693/36871
dc.language.isoEnglishen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttps://doi.org/10.1016/j.biomaterials.2016.08.045en_US
dc.source.titleBiomaterialsen_US
dc.subjectAnti-angiogenesisen_US
dc.subjectCorneal neovascularizationen_US
dc.subjectNeuropilin-1en_US
dc.subjectPeptide amphiphilesen_US
dc.subjectAmphiphilesen_US
dc.subjectBiocompatibilityen_US
dc.subjectCell proliferationen_US
dc.subjectComplex networksen_US
dc.subjectEndothelial cellsen_US
dc.subjectEpitopesen_US
dc.subjectNanofibersen_US
dc.subjectPolypeptidesen_US
dc.subjectAngiogenesisen_US
dc.subjectAnti-angiogenic activityen_US
dc.subjectNeo-vascularizationen_US
dc.subjectNeuropilin-1en_US
dc.subjectPeptide amphiphilesen_US
dc.subjectPeptide sequencesen_US
dc.subjectSelf-assembled peptidesen_US
dc.subjectSite of interestsen_US
dc.subjectPeptidesen_US
dc.subjectAnimal modelen_US
dc.subjectAnimal tissueen_US
dc.subjectAntiangiogenic activityen_US
dc.subjectIn vitro studyen_US
dc.subjectIn vivo studyen_US
dc.subjectPriority journalen_US
dc.subjectRaten_US
dc.subjectScanning transmission electron microscopyen_US
dc.subjectSutureen_US
dc.subjectTransmission electron microscopyen_US
dc.subjectAnimalen_US
dc.subjectChemistryen_US
dc.subjectCornea neovascularizationen_US
dc.subjectMetabolismen_US
dc.subjectPathologyen_US
dc.subjectSprague Dawley raten_US
dc.subjectTreatment outcomeen_US
dc.subjectAngiogenesis Inhibitorsen_US
dc.titleInhibition of VEGF mediated corneal neovascularization by anti-angiogenic peptide nanofibersen_US
dc.typeArticleen_US

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