A portable microfluidic platform for rapid determination of microbial load and somatic cell count in milk

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buir.contributor.authorÇetin, Barbaros
dc.citation.issueNumber3en_US
dc.citation.volumeNumber21en_US
dc.contributor.authorDüven, G.en_US
dc.contributor.authorÇetin, Barbarosen_US
dc.contributor.authorKurtuldu, H.en_US
dc.contributor.authorTiryaki-Gündüz, G.en_US
dc.contributor.authorTavman, Ş.en_US
dc.contributor.authorKışla, D.en_US
dc.date.accessioned2020-02-03T12:41:38Z
dc.date.available2020-02-03T12:41:38Z
dc.date.issued2019
dc.departmentDepartment of Mechanical Engineeringen_US
dc.description.abstractMicrofluidics systems that have been emerged in the last 20 years and used for processing the fluid in a microchannel structure at microliter levels are alternative to the conventional methods. The objective of the study is to develop a microfluidic platform for determination of the microbial load and the number of somatic cells in milk. For this purpose, a polydimethylsiloxane (PDMS) chip with a channel size of 300 μm × 60 μm was produced. Cells/bacteria labeled with fluorescent stain in milk were counted with the proposed microfluidic platform and the results were compared with the reference cell concentration/the bacterial counts by conventional method. It was found that our platform could count somatic and bacterial cells with an accuracy above 80% in 20 min run for each analysis. The portable overall platform has an overall dimension of 25x25x25 cm and weighs approximately 9 kg.en_US
dc.description.provenanceSubmitted by Zeynep Aykut (zeynepay@bilkent.edu.tr) on 2020-02-03T12:41:38Z No. of bitstreams: 1 A_portable_microfluidic_platform_for_rapid_determination_of_microbial_load_and_somatic_cell_count_in_milk.pdf: 843843 bytes, checksum: 141de17c00d4b4543bad836b4b104757 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-02-03T12:41:38Z (GMT). No. of bitstreams: 1 A_portable_microfluidic_platform_for_rapid_determination_of_microbial_load_and_somatic_cell_count_in_milk.pdf: 843843 bytes, checksum: 141de17c00d4b4543bad836b4b104757 (MD5) Previous issue date: 2019en
dc.identifier.doi10.1007/s10544-019-0407-8en_US
dc.identifier.issn1387-2176
dc.identifier.urihttp://hdl.handle.net/11693/53001
dc.language.isoEnglishen_US
dc.publisherSpringeren_US
dc.relation.isversionofhttps://dx.doi.org/10.1007/s10544-019-0407-8en_US
dc.source.titleBiomedical Microdevicesen_US
dc.subjectBacteria counten_US
dc.subjectMicrofluidicsen_US
dc.subjectQuality of milken_US
dc.subjectSomatic cell counten_US
dc.titleA portable microfluidic platform for rapid determination of microbial load and somatic cell count in milken_US
dc.typeArticleen_US

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