Portable microfluidic integrated plasmonic platform for pathogen detection

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dc.citation.epage9en_US
dc.citation.spage1en_US
dc.citation.volumeNumber5en_US
dc.contributor.authorTokel, O.en_US
dc.contributor.authorYildiz, U. H.en_US
dc.contributor.authorInci, F.en_US
dc.contributor.authorDurmus, N. G.en_US
dc.contributor.authorEkiz, O. O.en_US
dc.contributor.authorTurker, B.en_US
dc.contributor.authorCetin, C.en_US
dc.contributor.authorRao, S.en_US
dc.contributor.authorSridhar, K.en_US
dc.contributor.authorNatarajan, N.en_US
dc.contributor.authorShafiee, H.en_US
dc.contributor.authorDana, A.en_US
dc.contributor.authorDemirci, U.en_US
dc.date.accessioned2016-02-08T09:57:20Z
dc.date.available2016-02-08T09:57:20Z
dc.date.issued2015en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractTimely detection of infectious agents is critical in early diagnosis and treatment of infectious diseases. Conventional pathogen detection methods, such as enzyme linked immunosorbent assay (ELISA), culturing or polymerase chain reaction (PCR) require long assay times, and complex and expensive instruments, which are not adaptable to point-of-care (POC) needs at resource-constrained as well as primary care settings. Therefore, there is an unmet need to develop simple, rapid, and accurate methods for detection of pathogens at the POC. Here, we present a portable, multiplex, inexpensive microfluidic-integrated surface plasmon resonance (SPR) platform that detects and quantifies bacteria, i.e., Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) rapidly. The platform presented reliable capture and detection of E. coli at concentrations ranging from ∼105 to 3.2 × 107 CFUs/mL in phosphate buffered saline (PBS) and peritoneal dialysis (PD) fluid. The multiplexing and specificity capability of the platform was also tested with S. aureus samples. The presented platform technology could potentially be applicable to capture and detect other pathogens at the POC and primary care settings. © 2015, Nature Publishing Group. All rights reserved.en_US
dc.identifier.doi10.1038/srep09152en_US
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11693/22236
dc.language.isoEnglishen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttps://doi.org/10.1038/srep09152en_US
dc.source.titleScientific Reportsen_US
dc.subjectBiotechnologyen_US
dc.subjectHealth careen_US
dc.subjectNanobiotechnologyen_US
dc.titlePortable microfluidic integrated plasmonic platform for pathogen detectionen_US
dc.typeArticleen_US

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Institute of Materials Science and Nanotechnology (UNAM)