Bioactive peptide functionalized aligned cyclodextrin nanofibers for neurite outgrowth

buir.contributor.authorUyar, Tamer
buir.contributor.authorGüler, Mustafa O.
buir.contributor.orcidUyar, Tamer|0000-0002-3989-4481
dc.citation.epage524en_US
dc.citation.issueNumber3en_US
dc.citation.spage517en_US
dc.citation.volumeNumber5en_US
dc.contributor.authorHamsici, S.en_US
dc.contributor.authorCinar, G.en_US
dc.contributor.authorCelebioglu A.en_US
dc.contributor.authorUyar, Tameren_US
dc.contributor.authorTekinay, A. B.en_US
dc.contributor.authorGüler, Mustafa O.en_US
dc.date.accessioned2018-04-12T11:07:09Z
dc.date.available2018-04-12T11:07:09Z
dc.date.issued2017en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractGuidance of neurite extension and establishment of neural connectivity hold great importance for neural tissue regeneration and neural conduit implants. Although bioactive-epitope functionalized synthetic or natural polymeric materials have been proposed for the induction of neural regeneration, chemical modifications of these materials for neural differentiation still remain a challenge due to the harsh conditions of chemical reactions, along with non-homogeneous surface modifications. In this study, a facile noncovalent functionalization method is proposed by exploiting host-guest interactions between an adamantane-conjugated laminin derived bioactive IKVAV epitope and electrospun cyclodextrin nanofibers (CDNFs) to fabricate implantable scaffolds for peripheral nerve regeneration. While electrospun CDNFs introduce a three-dimensional biocompatible microenvironment to promote cellular viability and adhesion, the bioactive epitopes presented on the surface of electrospun CDNFs guide the cellular differentiation of PC-12 cells. In addition to materials synthesis and smart functionalization, physical alignment of the electrospun nanofibers guides the cells for enhanced differentiation. Cells cultured on aligned and IKVAV functionalized electrospun CDNFs had significantly higher expression of neuron-specific βIII-tubulin and synaptophysin. The neurite extension is also higher on the bioactive aligned scaffolds compared to random and non-functionalized electrospun CDNFs. Both chemical and physical cues were utilized for an effective neuronal differentiation process. © The Royal Society of Chemistry.en_US
dc.description.provenanceMade available in DSpace on 2018-04-12T11:07:09Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 179475 bytes, checksum: ea0bedeb05ac9ccfb983c327e155f0c2 (MD5) Previous issue date: 2017en
dc.identifier.doi10.1039/c6tb02441fen_US
dc.identifier.issn2050-7518
dc.identifier.urihttp://hdl.handle.net/11693/37246
dc.language.isoEnglishen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.isversionofhttps://doi.org/10.1039/c6tb02441fen_US
dc.source.titleJournal of Materials Chemistry Ben_US
dc.subjectBiocompatibilityen_US
dc.subjectChemical modificationen_US
dc.subjectCyclodextrinsen_US
dc.subjectEpitopesen_US
dc.subjectNanofibersen_US
dc.subjectNeuronsen_US
dc.subjectSurface treatmenten_US
dc.subjectCellular differentiationen_US
dc.subjectEnhanced differentiationsen_US
dc.subjectHost guest interactionsen_US
dc.subjectNeural differentiationsen_US
dc.subjectNeural tissue regenerationen_US
dc.subjectNeuronal differentiationen_US
dc.subjectNon-covalent functionalizationen_US
dc.subjectPeripheral nerve regenerationen_US
dc.subjectTissue regenerationen_US
dc.titleBioactive peptide functionalized aligned cyclodextrin nanofibers for neurite outgrowthen_US
dc.typeArticleen_US

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