Diabetic wound regeneration using heparin-mimetic peptide amphiphile gel in db/db mice

Date
2017
Advisor
Instructor
Source Title
Biomaterials Science
Print ISSN
2047-4830
Electronic ISSN
Publisher
Royal Society of Chemistry
Volume
5
Issue
7
Pages
1293 - 1303
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Abstract

There is an urgent need for more efficient treatment of chronic wounds in diabetic patients especially with a high risk of leg amputation. Biomaterials capable of presenting extracellular matrix-mimetic signals may assist in the recovery of diabetic wounds by creating a more conducive environment for blood vessel formation and modulating the immune system. In a previous study, we showed that glycosaminoglycan-mimetic peptide nanofibers are able to increase the rate of closure in STZ-induced diabetic rats by induction of angiogenesis. The present study investigates the effect of a heparin-mimetic peptide amphiphile (PA) nanofiber gel on full-thickness excisional wounds in a db/db diabetic mouse model, with emphasis on the ability of the PA nanofiber network to regulate angiogenesis and the expression of pro-inflammatory cytokines. Here, we showed that the heparin-mimetic PA gel can support tissue neovascularization, enhance the deposition of collagen and expression of alpha-smooth muscle actin (α-SMA), and eliminate the sustained presence of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in the diabetic wound site. As the absence of neovascularization and overexpression of pro-inflammatory markers are a hallmark of diabetes and interfere with wound recovery by preventing the healing process, the heparin-mimetic PA treatment is a promising candidate for acceleration of diabetic wound healing by modulating angiogenesis and local immune response. © 2017 The Royal Society of Chemistry.

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Keywords
Amphiphiles, Blood vessels, Enzyme activity, Immune system, Mammals, Nanofibers, Patient treatment, Peptides, Polysaccharides, Proteins, Efficient treatment, Extracellular matrices, Glycosaminoglycans, Neo-vascularization, Peptide amphiphiles, Pro-inflammatory cytokines, Smooth muscle actins, Tumor necrosis factor alpha, Biomimetics, Alpha smooth muscle actin, Collagen, Collagen type 1, Collagen type 2, Collagen type 3, Heparin derivative, Heparin mimetic peptide amphiphile nanofiber, Pathophysiology, Oligopeptides, Vascular Endothelial Growth Factor A, Wound Healing
Citation
Published Version (Please cite this version)