Supramolecular presentation of bioinstructive peptides on soft multilayered nanobiomaterials stimulates neurite outgrowth

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buir.contributor.authorSever-Bahçekapılı, Melike
buir.contributor.orcidSever-Bahçekapılı, Melike|0000-0002-0592-9666
dc.citation.epage5024en_US
dc.citation.issueNumber14
dc.citation.spage5012
dc.contributor.authorLopes, M.
dc.contributor.authorTorrado, M.
dc.contributor.authorBarth, D.
dc.contributor.authorSantos, S. D.
dc.contributor.authorSever-Bahçekapılı, Melike
dc.contributor.authorTekinay, A. B.
dc.contributor.authorGüler, M. O.
dc.contributor.authorCleymand, F.
dc.contributor.authorPêgo, A. P.
dc.contributor.authorBorges, J.
dc.contributor.authorMano, J. F.
dc.date.accessioned2024-03-11T06:26:28Z
dc.date.available2024-03-11T06:26:28Z
dc.date.issued2023-06-13
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)
dc.description.abstractPeptide amphiphiles (PAs) have emerged as effective molecular building blocks for creating self-assembling nanobiomaterials for multiple biomedical applications. Herein, we report a straightforward approach to assemble soft bioinstructive platforms to recreate the native neural extracellular matrix (ECM) aiming for neuronal regeneration based on the electrostatic-driven supramolecular presentation of laminin-derived IKVAV-containing self-assembling PA (IKVAV-PA) on biocompatible multilayered nanoassemblies. Spectroscopic and microscopic techniques show that the co-assembly of positively charged low-molecular-weight IKVAV-PA with oppositely charged high-molecular-weight hyaluronic acid (HA) triggers the formation of ordered β-sheet structures denoting a one-dimensional nanofibrous network. The successful functionalization of poly(L-lysine)/HA layer-by-layer nanofilms with an outer positively charged layer of self-assembling IKVAV-PA is demonstrated by the quartz crystal microbalance with dissipation monitoring and the nanofibrous morphological properties revealed by atomic force microscopy. The bioactive ECM-mimetic supramolecular nanofilms promote the enhancement of primary neuronal cells’ adhesion, viability, and morphology when compared to the PA without the IKVAV sequence and PA-free biopolymeric multilayered nanofilms, and stimulate neurite outgrowth. The nanofilms hold great promise as bioinstructive platforms for enabling the assembly of customized and robust multicomponent supramolecular biomaterials for neural tissue regeneration.
dc.description.provenanceMade available in DSpace on 2024-03-11T06:26:28Z (GMT). No. of bitstreams: 1 Supramolecular_presentation_of_bioinstructive_peptides_on_soft_multilayered_nanobiomaterials_stimulates_neurite_outgrowth.pdf: 3666859 bytes, checksum: cbca9377e30ed4314340f41e06234a2f (MD5) Previous issue date: 2023-06-13en
dc.identifier.doi10.1039/d3bm00438d
dc.identifier.eissn2047-4849
dc.identifier.issn2047-4830
dc.identifier.urihttps://hdl.handle.net/11693/114469
dc.language.isoEnglish
dc.publisherRoyal Society of Chemistry
dc.relation.isversionofhttps://dx.doi.org/10.1039/d3bm00438d
dc.source.titleBiomaterials Science
dc.titleSupramolecular presentation of bioinstructive peptides on soft multilayered nanobiomaterials stimulates neurite outgrowth
dc.typeArticle

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