Peptide nanofiber scaffolds for multipotent stromal cell culturing

Date
2013
Advisor
Instructor
Source Title
Methods in Molecular Biology
Print ISSN
10643745
Electronic ISSN
Publisher
Humana Press Inc.
Volume
1058
Issue
Pages
61 - 76
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Abstract

Self-assembled peptide nanofibers are versatile materials providing suitable platforms for regenerative medicine applications. This chapter describes the use of peptide nanofibers as extracellular matrix mimetic scaffolds for two-dimensional (2D) and three-dimensional (3D) multipotent stromal cell culture systems and procedures for in vitro experiments using these scaffolds. Preparation of 2D and 3D peptide nanofiber scaffolds and cell culturing procedures are presented as part of in vitro experiments including cell adhesion, viability, and spreading analysis. Analysis of cellular differentiation on peptide nanofiber scaffolds is described through immunocytochemistry, qRT-PCR, and other biochemical experiments towards osteogenic and chondrogenic lineage. © Springer Science+Business Media New York 2013.

Course
Other identifiers
Book Title
Keywords
Peptide nanofibers, Self-assembly, Hydrogels, Multipotent stem cells, Nanofiber
Citation
Published Version (Please cite this version)