Engineered bacteria with genetic circuits accumulating nanomagnets as MRI contrast agents

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buir.contributor.authorYavuz, Merve
buir.contributor.authorÜtkür, Mustafa
buir.contributor.authorKehribar, Ebru Şahin
buir.contributor.authorYağız, Ecrin
buir.contributor.authorSarıtaş, Emine Ülkü
buir.contributor.authorŞeker, Urartu Özgür Şafak
buir.contributor.orcidYavuz, Merve|0000-0003-1835-0503
buir.contributor.orcidÜtkür, Mustafa|0000-0002-2521-9151
buir.contributor.orcidKehribar, Ebru Şahin|0000-0003-2951-4390
buir.contributor.orcidYağız, Ecrin|0000-0001-8113-2192
buir.contributor.orcidSarıtaş, Emine Ülkü|0000-0001-8551-1077
buir.contributor.orcidŞeker, Urartu Özgür Şafak|0000-0002-5272-1876
dc.citation.epage2200537-11en_US
dc.citation.issueNumber26en_US
dc.citation.spage2200537-1en_US
dc.citation.volumeNumber18en_US
dc.contributor.authorYavuz, Merve
dc.contributor.authorÜtkür, Mustafa
dc.contributor.authorKehribar, Ebru Şahin
dc.contributor.authorYağız, Ecrin
dc.contributor.authorSarıtaş, Emine Ülkü
dc.contributor.authorŞeker, Urartu Özgür Şafak
dc.date.accessioned2023-02-14T11:50:33Z
dc.date.available2023-02-14T11:50:33Z
dc.date.issued2022-01-25
dc.departmentDepartment of Electrical and Electronics Engineeringen_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentInterdisciplinary Program in Neuroscience (NEUROSCIENCE)en_US
dc.departmentNational Magnetic Resonance Research Center (UMRAM)en_US
dc.description.abstractThe demand for highly efficient cancer diagnostic tools increases alongside the high cancer incidence nowadays. Moreover, there is an imperative need for novel cancer treatment therapies that lack the side effects of conventional treatment options. Developments in this aspect employ magnetic nanoparticles (MNPs) for biomedical applications due to their stability, biocompatibility, and magnetic properties. Certain organisms, including many bacteria, can synthesize magnetic nanocrystals, which help their spatial orientation and survival by sensing the earth's geomagnetic field. This work aims to convert Escherichia coli to accumulate magnetite, which can further be coupled with drug delivery modules. The authors design magnetite accumulating bacterial machines using genetic circuitries hiring Mms6 with iron-binding activity and essential in magnetite crystal formation. The work demonstrates that the combinatorial effect of Mms6 with ferroxidase, iron transporter protein, and material binding peptide enhances the paramagnetic behavior of the cells in magnetic resonance imaging (MRI) measurements. Cellular machines are also engineered to display Mms6 peptide on the cell surface via an autotransporter protein that shows augmented MRI performance. The findings are promising for endowing a probiotic bacterium, able to accumulate magnetite intracellularly or extracellularly, serving as a theranostics agent for cancer diagnostics via MRI scanning and hyperthermia treatment. © 2022 Wiley-VCH GmbH.en_US
dc.description.provenanceSubmitted by Evrim Ergin (eergin@bilkent.edu.tr) on 2023-02-14T11:50:33Z No. of bitstreams: 1 Engineered_bacteria_with_genetic_circuits_accumulating_nanomagnets_as_MRI_contrast_agents.pdf: 5705578 bytes, checksum: 858dd3a05b273c3dbf970612e346eb1f (MD5)en
dc.description.provenanceMade available in DSpace on 2023-02-14T11:50:33Z (GMT). No. of bitstreams: 1 Engineered_bacteria_with_genetic_circuits_accumulating_nanomagnets_as_MRI_contrast_agents.pdf: 5705578 bytes, checksum: 858dd3a05b273c3dbf970612e346eb1f (MD5) Previous issue date: 2022-01-25en
dc.identifier.doi10.1002/smll.202200537en_US
dc.identifier.eissn613-6829
dc.identifier.issn1613-6810
dc.identifier.urihttp://hdl.handle.net/11693/111261
dc.language.isoEnglishen_US
dc.publisherWileyen_US
dc.relation.isversionofhttps://doi.org/10.1002/smll.202200537en_US
dc.source.titleSmallen_US
dc.subjectGenetic circuitsen_US
dc.subjectIron oxide nanoparticlesen_US
dc.subjectMagnetic bacteriaen_US
dc.subjectMagnetic resonance imaging contrast agentsen_US
dc.subjectNanomagnetsen_US
dc.subjectSynthetic biologyen_US
dc.titleEngineered bacteria with genetic circuits accumulating nanomagnets as MRI contrast agentsen_US
dc.typeArticleen_US

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