In situ synthesis of biomolecule encapsulated gold-cross-linked poly(ethylene glycol) nanocomposite as biosensing platform: A model study

Date
2010
Advisor
Instructor
Source Title
Bioelectrochemistry
Print ISSN
1878-562X
Electronic ISSN
Publisher
Elsevier BV
Volume
79
Issue
2
Pages
211 - 217
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Abstract

In situ synthesis of poly(ethylene glycol) (PEG) hydrogels containing gold nanoparticles(AuNPs) and glucose oxidase (GOx) enzyme by photo-induced electron transfer process was reported here and applied in electrochemical glucose biosensing as the model system. Newly designed bionanocomposite matrix by simple one-step fabrication offered a good contact between the active site of the enzyme and AuNPs inside the network that caused the promotion in the electron transfer properties that was evidenced by cyclic voltammetryas well as higher amperometric biosensing responses in comparing with response signals obtained from the matrix without AuNPs. As well as some parameters important in the optimization studies such as optimum pH, enzyme loading and AuNP amount, the analytical characteristics of the biosensor (AuNP/GOx) were examined by the monitoring of chronoamperometric response due to the oxygen consumption through the enzymatic reaction at − 0.7 V under optimized conditions at sodium acetate buffer (50 mM, pH 4.0) and the linear graph was obtained in the range of 0.1–1.0 mM glucose. The detection limit (LOD) of the biosensor was calculated as 0.06 mM by using the signal to noise ratio of 3. Moreover, the presence of AuNPs was visualized by TEM. Finally, the biosensor was applied for glucose analysis for some beverages and obtained data were compared with HPLC as the reference method to test the possible matrix effect due to the nature of the samples.

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Keywords
Bionanocomposite, Biosensor, Gold nanoparticles, Hydrogel, Photopolymerization, Active site, Amperometric, Bionanocomposite, Biosensing, Biosensing platforms, Chronoamperometric response, Detection limits, Electron transfer, Enzymatic reaction, Enzyme loading, Glucose analysis, Gold nanoparticle, Gold Nanoparticles, In-situ synthesis, Linear graph, matrix, Matrix effects, Model study, Model system, Optimization studies, Optimized conditions, Optimum pH, Oxygen consumption, Photo-induced electron transfer, Reference method, Response signal, Sodium-acetate buffer, TEM, Beverages, Cyclic voltammetry, Electron transitions, Enzymes, Ethylene, Ethylene glycol, Glucose, Glucose oxidase, Glucose sensors, Gold, Hydrogels, Matrix algebra, Nanoparticles, Optimization, Oxygen, Photopolymerization, Polyethylene glycols, Polyethylene oxides, Signal to noise ratio, Sodium, Synthesis (chemical), Biosensors, gold nanoparticle, macrogol, nanocomposite, oxygen, amperometry, article, biosensor, calculation, cross linking, cyclic potentiometry, encapsulation, enzyme active site, enzyme mechanism, high performance liquid chromatography, hydrogel, monitoring, oxygen consumption, particle size, pH, polymerization, signal noise ratio, synthesis, transmission electron microscopy, Beverages, Biosensing Techniques, Buffers, Chromatography, High Pressure Liquid, Electrochemistry, Electrodes, Glucose, Glucose Oxidase, Gold, Hydrogen-Ion Concentration, Limit of Detection, Metal Nanoparticles, Microscopy, Electron, Transmission, Nanocomposites, Oxidation-Reduction, Polyethylene Glycols, Sodium Acetate
Citation
Published Version (Please cite this version)