Browsing by Subject "cancer"

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Open Access
Applications of biomaterials in cancer diagnosis and treatment
(2013) Gözen, Damla
Cancer remains to be a major burden of disease worldwide, despite the significant increase in the number of studies that focus on the development of novel diagnostic and treatment strategies. Recently, important part of these studies use biomaterials and their biomedical applications have been investigated extensively, due to their biocompatibility. Among these biomaterials two of them, carbon nanotubes (CNT) and polymer hydrogels have gained great importance due to their unique physical and chemical properties. The current study proposes new approaches that take advantage of these two biomaterials which could be used in the treatment and diagnosis of hepatocellular carcinoma (HCC). We first proposed the usage of CNTs as novel diagnostic tools for the determination of the aggressiveness of HCC. Two cell lines with different epithelial-to-mesenchymal (EMT) status, HUH7 and Snu182 were used and their attachment features on patterned CNT surfaces were compared. Our SEM images and MTT results revealed that the cells with epithelial phenotype (HUH7) attach and proliferate more on CNTs than the cells with mesenchymal phenotype (Snu182) which makes these surfaces promising diagnostic tools to differentiate HCC according to their aggressiveness. Secondly, polymer hydrogels with Dox release were suggested to be promising therapeutics to cure HCC. Our cell viability and cytotoxicity tests showed the inhibition of the proliferation of HCC line, SNU398 in the presence of drug-releasing hydrogels. This suggests the usage of hydrogels as drug delivery vehicles to have enhanced therapeutic efficacies in the HCC therapies.
Open Access
The cholesterol transporter ABCG1 links cholesterol homeostasis and tumour immunity
(Nature Publishing Group, 2015) Sag, D.; Cekic, C.; Wu, R.; Linden J.; Hedrick, C.C.
ATP-binding cassette transporter G1 (ABCG1) promotes cholesterol efflux from cells and regulates intracellular cholesterol homeostasis. Here we demonstrate a role of ABCG1 as a mediator of tumour immunity. Abcg1-/- mice have dramatically suppressed subcutaneous MB49-bladder carcinoma and B16-melanoma growth and prolonged survival. We show that reduced tumour growth in Abcg1-/- mice is myeloid cell intrinsic and is associated with a phenotypic shift of the macrophages from a tumour-promoting M2 to a tumour-fighting M1 within the tumour. Abcg1-/- macrophages exhibit an intrinsic bias towards M1 polarization with increased NF-κB activation and direct cytotoxicity for tumour cells in vitro. Overall, our study demonstrates that the absence of ABCG1 inhibits tumour growth through modulation of macrophage function within the tumour, and illustrates a link between cholesterol homeostasis and cancer. © 2015 Macmillan Publishers Limited. All rights reserved.