Browsing by Subject "Functionalized curli protein"

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Open Access
Synthetic cellular systems for whole cell biocatalysis
(2017-09) Apaydın, Onur
Synthetic biology is a field utilizing basic science and engineering approaches to create novel synthetic systems. Biocatalysis is one of those already existing processes which was reviewed intensely due to its advantages of using enzymes as catalysts. It is efficient, requires less additional reagents compared to chemical transformation methods, and it is environment friendly. Due to selectivity of enzymes it is easier to separate products. Enzymes are capable of carrying out many basic and complex reactions however some common problems occur in most strategies due to the nature of enzymes and mostly requirement of purification of the enzymes. Major issues are longevity-sustainability of the enzymes, modularity of the system, and yield of the enzymes. Thanks to the present advances in recombinant DNA technologies and discoveries in bacteria mechanisms like secretion, these pitfalls are addressable through Syntethic biology. We proposed a series of genetic circuits for the sustainability of biocatalysis systems by employing engineered bacterial biofilms. The final, biofilm proteins made nanofibers are protecting both cells and enzymes thus providing an environment fit for replenishment of the enzymes along with modularity to the system. Here we present two synthetic cellular systems utilizing engineered biofilms to address the issues of biocatalysis and we propose an RNA based synthetic regulatory component to increase the robustness of our systems.