Browsing by Subject "Chemical activation"

Now showing 1 - 6 of 6

Open Access
Antigenic GM3 lactone mimetic molecule integrated mannosylated glycopeptide nanofibers for the activation and maturation of dendritic cells
(American Chemical Society, 2017) Gunay, Gokhan; Ekiz, Melis Sardan; Ferhati, X.; Richichi, B.; Nativi, C.; Tekinay, Ayse B.; Güler, Mustafa O.
The ability of dendritic cells to coordinate innate and adaptive immune responses makes them essential targets for vaccination strategies. Presentation of specific antigens by dendritic cells is required for the activation of the immune system against many pathogens and tumors, and nanoscale materials can be functionalized for active targeting of dendritic cells. In this work, we integrated an immunogenic, carbohydrate melanoma-associated antigen-mimetic GM3-lactone molecule into mannosylated peptide amphiphile nanofibers to target dendritic cells through DC-SIGN receptor. Based on morphological and functional analyses, when dendritic cells were treated with peptide nanofiber carriers, they showed significant increase in antigen internalization and a corresponding increase in the surface expression of the activation and maturation markers CD86, CD83 and HLA-DR, in addition to exhibiting a general morphology consistent with dendritic cell maturation. These results indicate that mannosylated peptide amphiphile nanofiber carriers are promising candidates to target dendritic cells for antigen delivery. © 2017 American Chemical Society.
Open Access
Encapsulation of two different TLR ligands into liposomes confer protective immunity and prevent tumor development
(Elsevier B.V., 2017) Bayyurt, B.; Tincer, G.; Almacioglu, K.; Alpdundar, E.; Gursel, M.; Gursel, I.
Nucleic acid-based Toll-like receptor (TLR) ligands are promising adjuvants and immunotherapeutic agents. Combination of TLR ligands potentiates immune response by providing synergistic immune activity via triggering different signaling pathways and may impact antigen dependent T-cell immune memory. However, their short circulation time due to nuclease attack hampers their clinical performance. Liposomes offer inclusion of protein and nucleic acid-based drugs with high encapsulation efficiency and drug loading. Furthermore, they protect cargo from enzymatic cleavage while providing stability, and enhancing biological activity. Herein, we aimed to develop a liposomal carrier system co-encapsulating TLR3 (polyinosinic-polycytidylic acid; poly(I:C)) and TLR9 (oligodeoxynucleotides (ODN) expressing unmethylated CpG motifs; CpG ODN) ligands as immunoadjuvants together with protein antigen. To demonstrate that this depot system not only induce synergistic innate immune activation but also boost antigen-dependent immune response, we analyzed the potency of dual ligand encapsulated liposomes in long-term cancer protection assay. Data revealed that CpG ODN and poly(I:C) co-encapsulation significantly enhanced cytokine production from spleen cells. Activation and maturation of dendritic cells as well as bactericidal potency of macrophages along with internalization capacity of ligands were elevated upon incubation with liposomes co-encapsulating CpG ODN and poly(I:C). Immunization with co-encapsulated liposomes induced OVA-specific Th1-biased immunity which persisted for eight months post-booster injection. Subsequent challenge with OVA-expressing tumor cell line, E.G7, demonstrated that mice immunized with liposomes co-encapsulating dual ligands had significantly slower tumor progression. Tumor clearance was dependent on OVA-specific cytotoxic memory T-cells. These results suggest that liposomes co-encapsulating TLR3 and TLR9 ligands and a specific cancer antigen could be developed as a preventive cancer vaccine. ï¿½ 2017 Elsevier B.V.
Open Access
Examination of gas and solid products during the preparation of activated carbon using phosphoric acid
(Academic Press, 2018) Yagmur, E.; Inal, I. I. G.; Gokce, Y.; Ghobadi, T. G. U.; Aktar, T.; Aktas, Z.
Activating agents play significant roles in the preparation of activated carbon (AC) from biomasses and their wastes, which are widely used in AC production. Application methods are also important for the production process. Products give remarkable ideas regarding the method and heat treatment process. The activated carbon was produced from waste tea in accordance with either the conventional method or microwave energy pretreated method using phosphoric acid (H3PO4) as activating agent. The yields of the activated carbons were 51.8% for conventional method and 46.0% for microwave pretreated method. The acid suppressed the formation of tar and promoted the amount of solid and aromatic structure accordance to sp2 hybridisation. Additionally, the waste tea was directly carbonised (without H3PO4) and the yield was 36.3%. Major gas (H2, CH4, C2H6, C2H4, CO2 and CO) products obtained during heat treatment process in a conventional furnace were examined in terms of quantity and quality. The solid products were characterised in terms of surface area, pore size and surface properties. The result of gas analysis showed that phosphoric acid affected formation of activated carbon mechanism and significant reactions occurred during microwave pretreatment process.
Open Access
Highly stable Megalopolis lignite based N and S self-doped hierarchically porous activated carbons for high performance supercapacitors and ash content effects on performance
(Elsevier, 2022-02) Oglou, Ramadan Chalil; Gokce, Y.; Yagmur, E.; Ghobadi, T.Gamze Ulusoy; Aktas, Z.
This study proposes that high value-added activated carbon (AC) is produced from lignite (low rank coal) and used as electrode material in supercapacitors. In addition, the effects of the ash content of the activated carbon on supercapacitor performance are determined. The ACs at a temperature of 800 °C are produced in three different impregnation ratios (IR) (IR: 1, 2 and 4: KOH/precursor) using KOH as the activation agent. While the surface area of the AC produced at IR4 is 2531.4 m2 g−1, it is determined as 1321.3 (IR1) and 1989.0 (IR2) m2 g−1 for the others. The pore size analysis of the samples shows that the mesopores are dominant in the IR1 (52.73%) and IR4 (88.74%) samples, while it is observed that the micropores are dominant in the IR2 (69.03%) sample. The IR4 sample gives excellent results. The cell specific capacitances of this sample are determined as 260.47 F g−1 (at 1 A g−1) and 150.3 F g−1 (at 50 A g−1). The amount of ash in the AC samples dramatically reduces the supercapacitor performances and significantly increases the resistances.
Open Access
Mechanochemical activation and patterning of an adhesive surface toward nanoparticle deposition
(American Chemical Society, 2015) Baytekin, H. T.; Baytekin, B.; Huda, S.; Yavuz, Z.; Grzybowski, B. A.
Mechanical pulling of adhesive tape creates radicals on the tapes surface. These radicals are capable of reducing metal salts to the corresponding metal nanoparticles. In this way, the mechanically activated tape can be decorated with various types of nanoparticles, including Au, Ag, Pd, or Cu. While retaining their mechanical properties and remaining "sticky, " the tapes can exhibit new properties derived from the presence of metal nanoparticles (e.g., bacteriostaticity, increased electrical conductivity). They can also be patterned with nanoparticles only at selective locations of mechanical activation.
Open Access
Tactile perception by friction induced vibrations
(2011) Fagiani, R.; Massi, F.; Chatelet, E.; Berthier, Y.; Akay, A.
When a finger moves to scan the surface of an object (haptic sensing), the sliding contact generates vibrations that propagate in the finger skin activating the receptors (mechanoreceptors) located in the skin, allowing the brain to identify objects and perceive information about their properties. The information about the surface of the object is transmitted through vibrations induced by friction between the skin and the object scanned by the fingertip. The mechanoreceptors transduce the stress state into electrical impulses that are conveyed to the brain. A clear understanding of the mechanisms of the tactile sensing is fundamental to numerous applications, like the development of artificial tactile sensors for intelligent prostheses or robotic assistants, and in ergonomics. While the correlation between surface roughness and tactile sensation has already been reported in literature, the vibration spectra induced by the finger-surface scanning and the consequent activation of the mechanoreceptors on the skin have received less attention. In this paper, frequency analysis of signals characterizing surface scanning is carried out to investigate the vibration spectrum measured on the finger and to highlight the changes shown in the vibration spectra as a function of characteristic contact parameters such as scanning speed, roughness and surface texture. An experimental set-up is developed to recover the vibration dynamics by detecting the contact force and the induced vibrations; the bench test has been designed to guarantee reproducibility of measurements at the low amplitude of the vibrations of interest, and to perform measurements without introducing external noise. Two different perception mechanisms, as a function of the roughness wavelength, have been pointed out. The spectrum of vibration obtained by scanning textiles has been investigated. © 2011 Elsevier Ltd. All rights reserved.