Browsing by Subject "Cancer cells"

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    Classification of human carcinoma cells using multispectral imagery
    (SPIE, 2016) Çınar, U.; Çetin, Y. Y.; Çetin-Atalay, R.; Çetin, A. Enis
    In this paper, we present a technique for automatically classifying human carcinoma cell images using textural features. An image dataset containing microscopy biopsy images from different patients for 14 distinct cancer cell line type is studied. The images are captured using a RGB camera attached to an inverted microscopy device. Texture based Gabor features are extracted from multispectral input images. SVM classifier is used to generate a descriptive model for the purpose of cell line classification. The experimental results depict satisfactory performance, and the proposed method is versatile for various microscopy magnification options.
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    IKBKE-driven_TPL2_and_MEK1_phosphorylations_sustain_constitutive_ERK1_2_activation_in_tumor_cells
    (EXCLI Journal, 2022-02-17) Göktuna, Serkan İsmail
    IKBKE have been associated with numerous cancers. As a result, IKBKE have emerged as potential target for cancer therapy. Accumulating evidence support that IKBKE orchestrate tumor cell survival in cancers. Here we evaluated the possible link between IKBKE and ERK phosphorylation. The effects of IKBKE silencing on MAPK activation in tumor vs. normal cells were evaluated via WB and RT-PCR. Ectopically expressed IKBKE, TPL2 or MEK1 constructs were used to examine the possible interactions among them via co-IP. In vitro kinase assays were performed to understand nature of the observed interactions. In tumors, IKBKE regulates MEK/ERK constitutive activations in vitro and in vivo. IKBKE and TPL2 physically interact and this interaction leads to TPL2 phosphorylation. We describe here a novel regulatory link between IKBKE and constitutive ERK1/2 activation in tumor cells. This new circuitry may be relevant for tumor cell survival in various malignancies.
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    ItemOpen Access
    Near-IR absorbing BODIPY derivatives as glutathione-activated photosensitizers for selective photodynamic action
    (Wiley-VCH Verlag, 2014) Turan, I. S.; Cakmak, F. P.; Yildirim, D. C.; Cetin Atalay, R.; Akkaya, E. U.
    Enhanced spatiotemporal selectivity in photonic sensitization of dissolved molecular oxygen is an important target for improving the potential and the practical applications of photodynamic therapy. Considering the high intracellular glutathione concentrations within cancer cells, a series of BODIPY-based sensitizers that can generate cytotoxic singlet oxygen only after glutathione-mediated cleavage of the electron-sink module were designed and synthesized. Cell culture studies not only validate our design, but also suggest an additional role for the relatively hydrophobic quencher module in the internalization of the photosensitizer.