Browsing by Subject "Biomedical engineering"

Now showing 1 - 8 of 8

Open Access
Akut koroner sendromların otomatik ST/T sınıflandırıcısı ile erken tanısı
(IEEE, 2014-10) Terzi, M. Begüm; Arıkan, Orhan; Abacı, A.; Candemir, M.; Dedoğlu, Mehmet
In patients with acute coronary syndrome, temporary chest pains together with changes in ECG ST segment and T wave occur shortly before the start of myocardial infarction. In order to diagnose acute coronary syndromes early, a new technique which detects changes in ECG ST/T sections is developed. As a result of implementing the developed technique to real ECG recordings, it is shown that the proposed technique provides reliable detections. Therefore, the developed technique is expected to provide early diagnosis of acute coronary syndromes which will lead to a significant decrease in heart failure and mortality rates. © 2014 IEEE.
Open Access
Cornea engineering on polyester carriers
(John Wiley & Sons, Inc., 2006) Zorlutuna, P.; Tezcaner, A.; Kiyat, I.; Aydınlı, Atilla; Hasirci, V.
In this study, biodegradable polyester based carriers were designed for tissue engineering of the epithelial and the stromal layers of the cornea, and the final construct was tested in vitro. In the construction of the epithelial layer, micropatterned films were prepared from blends of biodegradable and biocompatible polyesters of natural (PHBV) and synthetic (P(L/DL)LA) origin, and these films were seeded with D407 (retinal pigment epithelial) cells. To improve cell adhesion and growth, the films were coated with fibronectin. To serve as the stromal layer of the cornea, highly porous foams of P(L/DL)LA-PHBV blends were seeded with 3T3 fibroblasts. Cell numbers on the polyester carriers were significantly higher than those on the tissue culture polystyrene control. The cells and the carriers were characterized scanning electron micrographs showed that the foam was highly porous and the pores were interconnected. 3T3 Fibroblasts were distributed quite homogeneously at the seeding site, but probably because of the high thickness of the carrier (∼6 mm); they could not sufficiently populate the core (central parts of the foam) during the test duration. The D407 cells formed multilayers on the micropatterned polyester film. Immunohistochemical studies showed that the cells retained their phenotype during culturing; D407 cells formed tight junctions characteristic of epithelial cells, and 3T3 cells deposited collagen type I into the foams. On the basis of these results, we concluded that the micropatterned films and the foams made of P(L/DL)LA-PHBV blends have a serious potential as tissue engineering carriers for the reconstruction of the epithelial and stromal layers of the cornea.
Open Access
İki durumlu bir beyin bilgisayar arayüzünde özellik çıkarımı ve sınıflandırma
(IEEE, 2017-10) Altındiş, Fatih; Yılmaz, B.
Beyin bilgisayar arayüzü (BBA) teknolojisi motor nöronlarının özelliğini kaybeden ve hareket kabiliyeti kısıtlanmış ALS ve felçli hastalar gibi birçok kişinin dış dünya ile iletişimini sağlamaya yönelik kullanılmaktadır. Bu çalışmada, Avusturya’daki Graz Üniversitesi’nde alınmış EEG veri seti kullanılarak gerçek zamanlı EEG işleme simülasyonu ile motor hayal etme sınıflandırılması amaçlanmıştır. Bu veri setinde sağ el ya da sol elin hareket ettirilme hayali esnasında 8 kişiden alınmış iki kanallı EEG sinyalleri bulunmaktadır. Her katılımcıdan 60 sağ ve 60 sol olmak üzere toplamda 120 adet yaklaşık 9 saniyelik motor hayal etme deneme sinyali kayıt edilmiştir. Bu sinyaller filtrelemeye tabi tutulmuştur. Yirmi dört, 32 ve 40 elemanlı özellik vektörü bant geçiren filtreler kullanarak elde edilen göreceli güç değişim değerleridir (GGDD). Bu çalışmada, lineer diskriminant analizi (LDA), k en yakın komşular (KNN) ve destek vektör makinaları (SVM) ile sınıflandırma yapılmış, en iyi sınıflandırma performansının 24 değerli özellik vektörüyle ve LDA sınıflandırma yöntemiyle elde edildiği gösterilmiştir.
Open Access
An integrated microfluidic device for the sorting of yeast cells using image processing
(Nature Publishing Group, 2018) Yu, B. Y.; Elbüken, Çağlar; Shen, C.; Huissoon, J. P.; Ren, C. L.
The process of detection and separation of yeast cells based on their morphological characteristics is critical to the understanding of cell division cycles, which is of vital importance to the understanding of some diseases such as cancer. The traditional process of manual detection is usually tedious and inconsistent. This paper presents a microfluidic device integrated with microvalves for fluid control for the sorting of yeast cells using image processing algorithms and confirmation based on their fluorescent tag. The proposed device is completely automated, low cost and easy to implement in an academic research setting. Design details of the integrated microfluidic system are highlighted in this paper, along with experimental validation. Real time cell sorting was demonstrated with a cell detection rate of 12 cells per minute.
Open Access
Materials for articular cartilage regeneration
(Bentham Science Publishers B.V., 2012) Tombuloglu, Ayşegül; Tekinay, Ayşe B.; Güler, Mustafa O.
Many health problems remaining to be untreatable throughout the human history can be overcome by utilizing new biomedical materials. Healing cartilage defects is one of the problems causing significant health issue due to low regeneration capacity of the cartilage tissue. Scaffolds as three-dimensional functional networks provide promising tools for complete regeneration of the cartilage tissue. Diversity of materials and fabrication methods give rise to many forms of scaffolds including injectable and mechanically stable ones. Various approaches can be considered depending on the condition of cartilage defect. A scaffold should maintain tissue function within a short time, and should be easily applied in order to minimally harm the body. This review will cover several patents and other publications on materials for cartilage regeneration with an outlook on essential characteristics of materials and scaffolds.
Open Access
Oxygen plasma modification of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) film surfaces for tissue engineering purposes
(John Wiley & Sons, Inc., 2003) Hasirci, V.; Tezcaner, A.; Hasirci, N.; Süzer, Ş.
Plasma glow-discharge application is known as a technique to coat or modify the surfaces of various materials. In this study, the influence of oxygen rf-plasma treatment on surface and bulk properties of a biological polyester, poly(3-hydroxybutyrate-co-3-hydroxyvalerate), were studied by determining water content and water contact angle, and by using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). The plasma-treated films absorbed more water than the untreated film, and the absorbance increased with the total power applied. The water contact angles decreased and O/C atomic ratio increased on treatment, indicating that the material became more hydrophilic due to increases in the oxygen-containing functional groups on the surface of the polymer. A direct relation could be observed when the O/C ratio was plotted against the total power applied (treatment duration x treatment power). SEM revealed a visual record of surface modification, the extent of which increased with increased total power. It was thus possible to alter the surface chemistry and relevant properties of the polymer film using oxygen plasma as a tool.
Open Access
Parallel minimum norm solution of sparse block diagonal column overlapped underdetermined systems
(Association for Computing Machinery, 2017) Torun, F. S.; Manguoglu, M.; Aykanat, Cevdet
Underdetermined systems of equations in which the minimum norm solution needs to be computed arise in many applications, such as geophysics, signal processing, and biomedical engineering. In this article, we introduce a new parallel algorithm for obtaining the minimum 2-norm solution of an underdetermined system of equations. The proposed algorithm is based on the Balance scheme, which was originally developed for the parallel solution of banded linear systems. The proposed scheme assumes a generalized banded form where the coefficient matrix has column overlapped block structure in which the blocks could be dense or sparse. In this article, we implement the more general sparse case. The blocks can be handled independently by any existing sequential or parallel QR factorization library. A smaller reduced system is formed and solved before obtaining the minimum norm solution of the original system in parallel. We experimentally compare and confirm the error bound of the proposed method against the QR factorization based techniques by using true single-precision arithmetic. We implement the proposed algorithm by using the message passing paradigm. We demonstrate numerical effectiveness as well as parallel scalability of the proposed algorithm on both shared and distributed memory architectures for solving various types of problems. © 2017 ACM.
Open Access
Sheathless microflow cytometry using viscoelastic fluids
(Nature Publishing Group, 2017) Asghari, M.; Serhatlioglu, M.; Ortaç, B.; Solmaz, M. E.; Elbuken, C.
Microflow cytometry is a powerful technique for characterization of particles suspended in a solution. In this work, we present a microflow cytometer based on viscoelastic focusing. 3D single-line focusing of microparticles was achieved in a straight capillary using viscoelastic focusing which alleviated the need for sheath flow or any other actuation mechanism. Optical detection was performed by fiber coupled light source and photodetectors. Using this system, we present the detection of microparticles suspended in three different viscoelastic solutions. The rheological properties of the solutions were measured and used to assess the focusing performance both analytically and numerically. The results were verified experimentally, and it has been shown that polyethlyene oxide (PEO) and hyaluronic acid (HA) based sheathless microflow cytometer demonstrates similar performance to state-of-the art flow cytometers. The sheathless microflow cytometer was shown to present 780 particles/s throughput and 5.8% CV for the forward scatter signal for HA-based focusing. The presented system is composed of a single capillary to accommodate the fluid and optical fibers to couple the light to the fluid of interest. Thanks to its simplicity, the system has the potential to widen the applicability of microflow cytometers.