Browsing by Subject "Behçet's disease"
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Item Open Access Ant i-neuronal and stress-induced-phosphoprotein 1 antibodies in neuro-Behcet's disease(Elsevier, 2011-10-28) Vural, B.; Uğurel, E.; Tüzün, E.; Kürtüncü, M.; Zuliani, L.; Çavus, F.; İçoz, S.; Erdağ, E.; Gül, A.; Güre, A. O.; Vincent, A.; Özbek, U.; Eraksoy, M.; Demir, G. A.No disease-specific neuronal antibodies have so far been defined in neuro-Behçet's disease (NBD). Immunohistochemistry and immunocytochemistry studies showed antibodies to hippocampal and cerebellar molecular layers and the surface antigens of cultured hippocampal neurons in sera and/or cerebrospinal fluids (CSF) of 13 of 20 NBD and 6 of 20 BD patients but not in multiple sclerosis or headache controls. Screening with a protein macroarray led to identification of stress-induced-phosphoprotein-1 (STIP-1) as an antigenic target. High-titer STIP-1-antibodies were detected in 6 NBD patients' sera but not in controls. These results suggest that neuronal antibodies could be useful as diagnostic biomarkers in NBD. © 2011 Elsevier B.V.Item Open Access Circulating LL37 targets plasma extracellular vesicles to immune cells and intensifies Behçet's disease severity(Taylor and Francis, 2017-02) Kahraman, T.; Gucluler, G.; Simsek, I.; Yagci, F. C.; Yildirim, M.; Ozen, C.; Dinc, A.; Gursel, M.; Ikromzoda, L.; Sutlu, T.; Gay, S.; Gursel, I.Behçet's disease (BD) activity is characterised by sustained, over-exuberant immune activation, yet the underlying mechanisms leading to active BD state are poorly defined. Herein, we show that the human cathelicidin derived antimicrobial peptide LL37 associates with and directs plasma extracellular vesicles (EV) to immune cells, thereby leading to enhanced immune activation aggravating BD pathology. Notably, disease activity was correlated with elevated levels of circulating LL37 and EV plasma concentration. Stimulation of healthy PBMC with active BD patient EVs induced heightened IL1β, IFNα, IL6 and IP10 secretion compared to healthy and inactive BD EVs. Remarkably, when mixed with LL37, healthy plasma-EVs triggered a robust immune activation replicating the pathology inducing properties of BD EVs. The findings of this study could be of clinical interest in the management of BD, implicating LL37/EV association as one of the major contributors of BD pathogenesis.Item Open Access Mitochondrial carrier homolog 1 (Mtch1) antibodies in neuro-Behçet's disease(Elsevier, 2013) Vural, B.; Şehitoğlu, E.; Çavuş, F.; Yalçınkaya, N.; Haytural, H.; Küçükerden, M.; Ulusoy, C.; Uğurel, E.; Turan, S.; Bulut, L.; Türkoğlu, R.; Shugaiv, E.; Kürtüncü, M.; Atakan, S.; Güre, A. O.; Gül, A.; Eraksoy, M.; Demir, G. A.; Tüzün, E.Efforts for the identification of diagnostic autoantibodies for neuro-Behcet's disease (NBD) have failed. Screening of NBD patients' sera with protein macroarray identified mitochondrial carrier homolog 1 (Mtch1), an apoptosis-related protein, as a potential autoantigen. ELISA studies showed serum Mtch1 antibodies in 68 of 144 BD patients with or without neurological involvement and in 4 of 168 controls corresponding to a sensitivity of 47.2% and specificity of 97.6%. Mtch1 antibody positive NBD patients had more attacks, increased disability and lower serum nucleosome levels. Mtch1 antibody might be involved in pathogenic mechanisms of NBD rather than being a coincidental byproduct of autoinflammation. © 2013 Elsevier B.V.