Browsing by Subject "Attachment"
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Item Open Access Asialo-GM1 and asialo-GM2 are putative adhesion molecules for Moraxella catarrhalis(Springer-Verlag, 2002) Ahmed, K.; Suzuki, Y.; Miyamoto, D.; Nagatake, T.Moraxella catarrhalis is an important pathogen of respiratory and middle ear infections. We previously reported that the attachment of M. catarrhalis to pharyngeal epithelial cells is mediated by ganglioside M2 (GM2). Several sets of adhesins or receptors are involved in such attachment process. In this study, we used the same strains and similar bacterial culture conditions as those in our previous study, and demonstrated by thin layer chromatography that M. catarrhalis can also bind toasialo-GM1 (Gg4Cer) and asialo-GM2 (Gg3Cer). GalNAcβ1→4Galβ1 is a common sequence in both Gg4Cer and Gg3Cer, and in many respiratory bacteria, this sequence acts as a receptor for attachment to host cells. Treatment of human pharyngeal epithelial cells with anti-GM2 and anti-Gg4Cer antibodies significantlydecreased attachment of M. catarrhalis to these cells; however, treatment with anti-Gg3Cer antibody did not decrease M. catarrhalis attachment. Immunofluorescence microscopy revealed that human pharyngeal epithelial cells are positive for GM2 and Gg4Cer, but not for Gg3Cer. Our results indicate that Gg4Cer on human pharyngeal epithelial cells, and Gg3Cer, possibly on other cells, could serve as molecules for attachment of M. catarrhalis.Item Open Access The effects of video-feedback intervention to promote positive parenting and sensitive discipline for maternal sensitivity in Turkey(Turkish Psychological Association, 2020) Sümer, N.; Metin-Orta, İ.; Alsancak-Akbulut, C.; Salman-Engin, Selin; İlden-Koçkar, A.; Şahin-Acar, B.; Akkol-Solakoğlu, S.; Aran, Ö.; Çakır, D. S.; Kavaklı, B.; Memişoğlu-Şanlı, A.; Sağel-Çetiner, E.; Türe, D.; Üstünel, A. Ö.; Yaşar, B.The aim of the current study was to examine the effectiveness of a culturally sensitive adaptation of a Video-feedback Intervention to promote ‘Positive Parenting and Sensitive Discipline’ (VIPP-SD) in Turkey, and to assess its effectiveness on maternal sensitivity. The study included mothers of 9-33-months-old children, with a relatively lower SES, from Ankara and İstanbul (Nfemale = 48). The sample was randomly divided into two groups as control and intervention. Four thematic meetings regarding sensitivity and sensitive discipline were held with the 59 mothers who were in the intervention group. Through intervention sessions, mothers were shown and provided with feedback on short videos, in which they had been recorded in the previous session, interacting with their children. Maternal sensitivity was evaluated through the ‘Ainsworth Maternal Sensitivity Scale’ by coding the recorded video clips showing mother-child interactions. Maternal behaviors and attitudes towards discipline were assessed by the ‘Maternal Attitudes toward Sensitivity and Sensitive Discipline Scale’. The results showed that there was a significant increase in mothers’ observed maternal sensitivity and reported attitudes toward maternal sensitivity in the intervention group between pre-test and post-test assessments, whereas there was no significant change in the control group. There was no significant effect of the program on self-reported attitudes toward sensitive discipline. These results suggest that VIPPSD is effective in increasing maternal sensitivity in low-middle SES mothers in the relatively collectivistic culture of Turkey.Item Open Access Role of lipooligosaccharide in the attachment of Moraxella catarrhalis to human pharyngeal epithelial cells(Wiley, 2005) Akgul, G.; Erturk, A.; Turkoz, M.; Turan, T.; Ichinose, A.; Nagatake, T.; Ahmed, K.The goal of this study was to determine the role of lipooligosaccharide in the attachment of Moraxella catarrhalis to human pharyngeal epithelial cells. Strain 2951 and its Pk mutant strain 2951 galE were used in this study. This study suggests that the Pk epitope of LOS is not an adhesin for M. catarrhalis, but plays a crucial role by its surface charge in the initial stage of attachment.Item Open Access Sulfatide and its synthetic analogues recognition by Moraxella catarrhalis(Springer, 2006) Ozcelik, P.; Bezirci, F. B.; Suzuki, Y.; Uzawa, H.; Nishida, Y.; Kobayashi, K.; Suzuki, T.; Miyamoto, D.; Nagatake, T.; Ahmed, K.Moraxella catarrhalis is one of the major pathogens of respiratory and middle ear infections. Attachment of this bacterium to the surface of human pharyngeal epithelial cells is the first step in the pathogenesis of infections. This study revealed that sulfatide might act as a binding molecule for the attachment of M. catarrhalis to human pharyngeal epithelial cells. Furthermore, six different synthetic sulfatides were found to inhibit the attachment of M. catarrhalis significantly at an optimum concentration of 10 μg/ml. Synthetic sulfatides may have the potential to be used as a therapy to prevent M. catarrhalis infections.Item Open Access Sulfatide mediates attachment of Pseudomonas aeruginosa to human pharyngeal epithelial cells(Edizioni Medico Scientifiche, 2007) Yagci, A.; Yagci, T.; Sener, B.; Suziki, Y.; Ahmed, K.Pseudomonas aeruginosa infections are particularly common in people with cystic fibrosis and despite regular treatment with antibiotics, lung damage due to chronic infection with P. aeruginosa remains the major cause of death in those patients. In order to initiate an infection, P. aeruginosa needs contact with the respiratory epithelial surface and by means of its adhesins i.e., fimbria, hemagglutinins,etc., it recognizes and adheres to the corresponding epithelial receptors. We treated P. aeruginosa strains isolated from sputum of cystic fibrosis patients with several glycolipids such as sulfatide, sulfated ganglioside mixture (GM1a, GD1b, GT1b), asialo-GM1 and galactocerebrosides to determine their effect on attachment with pharyngeal epithelial cells. Sulfated ganglioside mixture and sulfatide inhibited the attachment of P. aeruginosa significantly, whereas asialo-GM1, Gal-Cer and sodium sulfite had no effect on attachment inhibition. This finding suggests that sulfated glycoconjugates found in the extracellular matrix, in mucus and on the surface of epithelial cells of human trachea and lung mediates attachment of P. aeruginosa.