Browsing by Author "Avadhani, A."
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Item Open Access Bio-inspired magnetic beads for isolation of sperm from heterogenous samples ın forensic applications(Elsevier BV, 2021-05) İnci, Fatih; Karaaslan, M. G.; Gupta, R.; Avadhani, A.; Öğüt, M. G.; Atila, E. E.; Duncan, G.; Klevan, L.; Demirci, U.Rapid and efficient processing of sexual assault evidence will accelerate forensic investigation and decrease casework backlogs. The standardized protocols currently used in forensic laboratories require the continued innovation to handle the increasing number and complexity of samples being submitted to forensic labs. Here, we present a new technique leveraging the integration of a bio-inspired oligosaccharide (i.e., Sialyl‐LewisX) with magnetic beads that provides a rapid, inexpensive, and easy-to-use strategy that can potentially be adapted with current differential extraction practice in forensics labs. This platform (i) selectively captures sperm; (ii) is sensitive within the forensic cut-off; (iii) provides a cost effective solution that can be automated with existing laboratory platforms; and (iv) handles small volumes of sample (∼200 μL). This strategy can rapidly isolate sperm within 25 minutes of total processing that will prepare the extracted sample for downstream forensic analysis and ultimately help accelerate forensic investigation and reduce casework backlogs.Item Open Access Enhancing the nanoplasmonic signal by a nanoparticle sandwiching strategy to detect viruses(Elsevier, 2020-05) Inci, Fatih; Karaaslan, M. G.; Mataji-Kojouri, A.; Saylan, Y.; Zeng, Y.; Avadhani, A.; Sinclair, R.; Lau, D. T.-Y.; Demirci, U.Nanoparticles that can assemble and bind selectively on surfaces in intricate geometries can trigger multiple plasmonic modalities and enable wide applications in agriculture such as pesticide monitoring, in medical imaging such as targeted cancer detection, in bioengineering such as biotarget detection and biosensing, and in healthcare such as selection of drugs and their binding kinetics. However, these particles mainly rely on binding of the target to a surface to create a plasmonic resonance and subsequent shifts by binding of biotargets, which limit the flexibility to control overall sensitivity. Here, we present an unconventional way that sandwiches a virus (i.e., Hepatitis B virus: HBV) topographically between two or more nanoparticles on the top and the bottom to create a double-step shifting effect amplifying the total resonance wavelength shift on the surface by 1.53 - 1.77 times that significantly enhances the sensitivity. We successfully applied this approach to an intact HBV sensing application, which accurately quantified the viral load. This method establishes a new nanoparticle-based sandwiched nanoplasmonic approach to detect and quantify viral load using two-step sensing with broad applications in biosensing.