Browsing by Author "İbrahimova, Vusala"

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    Nanoparticle labeling: a new era in vivo tracing the cells in liver studies
    (Elsevier, 2012-04) Akhan, Ece; Aydın, Muammer Merve; İbrahimova, Vusala; Buğdaycı, Kadir Emre; Tuncel, Dönüş; Akçalı, Kamil Can
    Tracking molecules in vivo has always been challenging and there is increasing demand for tools that allow researchers to trace especially during cell-based therapies. Conjugated polymer based water-dispersible nanoparticles (NP) represent a new class of probes for cell imaging and tracking because they offer high brightness, improved photostability, high fluorescent quantum yield and non-cytotoxicity comparing to conventional dyes. We aimed to develop a novel approach by using fluorescein emitting NP to label Mesenchymal Stem Cells (MSCs) and HUH7 cell lines and assess their fate in vivo in liver regeneration, liver fibrosis and xenografts.
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    Synthesis and characterization of water dispersible conjugated polymer nanoparticles
    (2011) İbrahimova, Vusala
    In this study, novel water dispersible conjugated polymer nanoparticles having various potential applications in the areas including biomedicine and photonics have been synthesized from blue, green and yellow light emitting conjugated polymers. Their sizes, morphology, surface charges and optical properties have been determined using various techniques. Cell viability of nanoparticles was tested in mesenchymal stem cells. For the synthesis of nanoparticles, first the following polymers carrying a number of different functional groups and based on derivatives of fluorene and benzothiodiazole monomers are designed and synthesized using the Suzuki coupling reactions: Poly[(9,9-bis{propeny}fluorenyl-2,7-diyl)-co-(9,9-dihexyl-9H-fluorene)] (P1), poly[(9,9-bis{carboxymethylsulfonyl-propyl}fluorenyl-2,7-diyl)-co-(9,9-dihexyl-9Hfluorene) (P2), poly[(9,9- bis{propeny}fluorenyl-2,7-diyl))-co-(1,4-benzo-{2,1,3}- thiodiazole)] (P3), poly[(9,9- bis{carboxymethylsulfonyl-propyl}fluorenyl-2,7-diyl)- co(1,4-benzo-{2,1,3}-thiodiazole)] (P4), poly[(9,9-bis{3-bromopropyl}fluorenyl- 2,7-diyl)-co-(1,4-benzo-{2,1,3}-thiodiazole)] (P5), poly[(9,9-bis{3- azidopropyl}fluorenyl-2,7-diyl)-co-(benzothiadiazole)] (P6). Polymers were characterized by using spectroscopic techniques such as 1H-NMR, FT-IR, UV-Vis, Fluorescence spectrophotometer and Gel Permeation Chromatography (GPC). Conjugated polymers carry functional groups on their side chains, such as azide and allyl groups that can be cross-linkable using UV light to form shape-persistent, stable nanoparticles. Nanoparticles were characterized by various techniques before and after UV-treatment. Their sizes and morphologies were determined by using dynamic light scattering measurements (DLS) and imaging techniques such as scanning electron microscopy (SEM) and atomic force microscopy (AFM). For optical characterization UV-vis, fluorescent spectroscopies and FT-IR were used. CNPs affect on cells shows their nontoxic and biocompatible properties which give opportunity to use them in cell imaging.