Molecular karyotyping of human hepatocellular carcinoma cell lines using single-nucleotide polymorphism arrays

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buir.advisorYakıcıer, M. Cengiz
dc.contributor.authorDemir, Kubilay
dc.date.accessioned2016-01-08T18:02:07Z
dc.date.available2016-01-08T18:02:07Z
dc.date.issued2007
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.descriptionAnkara : The Department of Moleculer Biology and Genetics and the Institute of Engineering and Science of Bilkent University, 2007.en_US
dc.descriptionThesis (Master's) -- Bilkent University, 2007.en_US
dc.descriptionIncludes bibliographical references leaves 83-110.en_US
dc.description.abstractHepatocellular carcinoma (HCC) etiology is genetically heterogeneous; multiple different mechanisms have been shown to promote hepatocarcinogenesis. However, chromosomal aberrations (CAs) and signaling pathways that they alter are still poorly understood. Changes in chromosome number (aneuploidies) or structural chromosomal aberrations, such as; amplifications, deletions, loss of heterozygosity and recessive mutations are important mechanisms for tumor evolution. Recently developed single nucleotide polymorphism (SNP) microarrays provide highthroughput quantitative and qualitative screening of genomic DNA with higher resolution compared to conventional methods such as fluorescent in situ hybridization (FISH) and comparative genomic hybridization (CGH). In cancer research, SNP arrays ease the screening of structural changes as well as aneuploidies with exact physical position. In the framework of this study, we aimed to detect DNA copy number alterations in a panel of 14 HCC cell lines. We screened all the autosomal chromosomes and the Xchromosome and found previously undescribed novel regions that harbor homozygous and hemizygous deletions at 13q12 and Xq21; amplifications at 8p23, 8q13, 8q24, 9p22- 21, 12p1, 14q12, 15q21, 16q23, 17p12-p11, 17q11, 22q11 and Xp22. In our knowledge, our results are the first comprehensive high-throughput screen of commonly used HCC cell lines.en_US
dc.description.degreeM.S.en_US
dc.description.statementofresponsibilityDemir, Kubilayen_US
dc.format.extentxiii, 110 leaves, illustrations, graphicsen_US
dc.identifier.urihttp://hdl.handle.net/11693/14560
dc.language.isoEnglishen_US
dc.publisherBilkent Universityen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject.lccWI735 .D46 2007en_US
dc.subject.lcshCarcinoma, Hepatocellular.en_US
dc.subject.lcshLiver cancer.en_US
dc.titleMolecular karyotyping of human hepatocellular carcinoma cell lines using single-nucleotide polymorphism arraysen_US
dc.typeThesisen_US
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Dept. of Molecular Biology and Genetics - Master's degree