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      Tenascin-C mimetic peptide nanofibers direct stem cell differentiation to osteogenic lineage

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      Author
      Sever, Melike
      Mammadov, Busra
      Güler, Mustafa O.
      Tekinay, Ayşe B.
      Date
      2014
      Source Title
      Biomacromolecules
      Print ISSN
      1525-7797
      Electronic ISSN
      1526-4602
      Publisher
      American Chemical Society
      Volume
      15
      Issue
      12
      Pages
      4480 - 4487
      Language
      English
      Type
      Article
      Item Usage Stats
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      Abstract
      Extracellular matrix contains various signals for cell surface receptors that regulate cell fate through modulation of cellular activities such as proliferation and differentiation. Cues from extracellular matrix components can be used for development of new materials to control the stem cell fate. In this study, we achieved control of stem cell fate toward osteogenic commitment by using a single extracellular matrix element despite the contradictory effect of mechanical stiffness. For this purpose, we mimicked bone extracellular matrix by incorporating functional sequence of fibronectin type III domain from native tenascin-C on self-assembled peptide nanofibers. When rat mesenchymal stem cells (rMSCs) were cultured on these peptide nanofibers, alkaline phosphatase (ALP) activity and alizarin red staining indicated osteogenic differentiation even in the absence of osteogenic supplements. Moreover, expression levels of osteogenic marker genes were significantly enhanced revealed by quantitative real-time polymerase chain reaction (qRT-PCR), which showed the remarkable bioactive role of this nanofiber system on osteogenic differentiation. Overall, these results showed that tenascin-C mimetic peptides significantly enhanced the attachment, proliferation, and osteogenic differentiation of rMSCs even in the absence of any external bioactive factors and regardless of the suitable stiff mechanical properties normally required for osteogenic differentiation. Thus, these peptide nanofibers provide a promising new platform for bone regeneration. © 2014 American Chemical Society.
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      http://hdl.handle.net/11693/24814
      Published Version (Please cite this version)
      http://dx.doi.org/10.1021/bm501271x
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      • Aysel Sabuncu Brain Research Center (BAM) 197
      • Institute of Materials Science and Nanotechnology (UNAM) 1845
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