Tenascin-C mimetic peptide nanofibers direct stem cell differentiation to osteogenic lineage

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buir.contributor.authorSever, Melike
buir.contributor.authorMammadov, Busra
buir.contributor.authorGüler, Mustafa O.
buir.contributor.authorTekinay, Ayşe B.
dc.citation.epage4487en_US
dc.citation.issueNumber12en_US
dc.citation.spage4480en_US
dc.citation.volumeNumber15en_US
dc.contributor.authorSever, Melikeen_US
dc.contributor.authorMammadov, Busraen_US
dc.contributor.authorGüler, Mustafa O.en_US
dc.contributor.authorTekinay, Ayşe B.en_US
dc.date.accessioned2016-02-08T10:34:46Z
dc.date.available2016-02-08T10:34:46Z
dc.date.issued2014en_US
dc.departmentAysel Sabuncu Brain Research Center (BAM)en_US
dc.description.abstractExtracellular matrix contains various signals for cell surface receptors that regulate cell fate through modulation of cellular activities such as proliferation and differentiation. Cues from extracellular matrix components can be used for development of new materials to control the stem cell fate. In this study, we achieved control of stem cell fate toward osteogenic commitment by using a single extracellular matrix element despite the contradictory effect of mechanical stiffness. For this purpose, we mimicked bone extracellular matrix by incorporating functional sequence of fibronectin type III domain from native tenascin-C on self-assembled peptide nanofibers. When rat mesenchymal stem cells (rMSCs) were cultured on these peptide nanofibers, alkaline phosphatase (ALP) activity and alizarin red staining indicated osteogenic differentiation even in the absence of osteogenic supplements. Moreover, expression levels of osteogenic marker genes were significantly enhanced revealed by quantitative real-time polymerase chain reaction (qRT-PCR), which showed the remarkable bioactive role of this nanofiber system on osteogenic differentiation. Overall, these results showed that tenascin-C mimetic peptides significantly enhanced the attachment, proliferation, and osteogenic differentiation of rMSCs even in the absence of any external bioactive factors and regardless of the suitable stiff mechanical properties normally required for osteogenic differentiation. Thus, these peptide nanofibers provide a promising new platform for bone regeneration. © 2014 American Chemical Society.en_US
dc.identifier.doi10.1021/bm501271xen_US
dc.identifier.eissn1526-4602
dc.identifier.issn1525-7797
dc.identifier.urihttp://hdl.handle.net/11693/24814
dc.language.isoEnglishen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isversionofhttp://dx.doi.org/10.1021/bm501271xen_US
dc.source.titleBiomacromoleculesen_US
dc.titleTenascin-C mimetic peptide nanofibers direct stem cell differentiation to osteogenic lineageen_US
dc.typeArticleen_US
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Institute of Materials Science and Nanotechnology (UNAM)
Aysel Sabuncu Brain Research Center (BAM)