Multi-Domain Short Peptide Molecules for in Situ Synthesis and Biofunctionalization of Gold Nanoparticles for Integrin-Targeted Cell Uptake
Unal Gulsuner H.
ACS Applied Materials and Interfaces
American Chemical Society
Please cite this item using this persistent URLhttp://hdl.handle.net/11693/21892
We describe design and synthesis model of multidomain (modular) peptides (MDPs), which direct a reaction cascade coupling the synthesis and surface functionalization of gold nanoparticles (AuNPs) in a single step. The synthesis is achieved via simple mixing of the aqueous solutions of auric acid and MDPs at room temperature without the addition of any surfactants or toxic intermediate reagents. This method allows facile control over the nanoparticle size between ∼2-15 nm, which opens a practical window for biomedical applications. In contrast to the conventional citrate-mediated methods, peptide-mediated synthesis and stabilization provide increased colloidal stability to AuNPs. As a proof of this concept, we demonstrate active targeting of human breast adenocarcinoma cell line (MCF7) using the one-step-prepared engineered AuNPs. Overall, we propose a single-step, chemically greener, biologically safer method for the synthesis and surface functionalization of gold nanoparticles in a size-controlled manner. The chemical versatility of the MDP design broadens the applicability of this strategy, thereby emerging as a successful alternative for the currently available nanoparticle preparation technologies. (Chemical Presented). © 2015 American Chemical Society.
- Research Paper 
Showing items related by title, author, creator and subject.
Baytekin H.T.; Baytekin, B.; Huda, S.; Yavuz, Z.; Grzybowski, B.A. (American Chemical Society, 2015)Mechanical pulling of adhesive tape creates radicals on the tapes surface. These radicals are capable of reducing metal salts to the corresponding metal nanoparticles. In this way, the mechanically activated tape can be ...
One-step synthesis of size-tunable Ag nanoparticles incorporated in electrospun PVA/cyclodextrin nanofibers Celebioglu, A.; Aytac, Z.; Umu O.C.O.; Dana, A.; Tekinay, T.; Uyar, T. (2014)One-step synthesis of size-tunable silver nanoparticles (Ag-NP) incorporated into electrospun nanofibers was achieved. Initially, in situ reduction of silver salt (AgNO3) to Ag-NP was carried out in aqueous solution of ...
Üçüncü, E.; Özkan, A.D.; Kurşungöz, C.; Ülger, Z.E.; Ölmez, T.T.; Tekinay, T.; Ortaç, B.; Tunca, E. (Elsevier Ltd, 2014)The present study investigates and models the effect of laser ablated silver nanoparticles (AgNPs) on the development of the aquatic macrophyte Lemna minor. Toxic effects of five different AgNP concentrations (8, 16, 32, ...