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      A genetically engineered biofilm material for SARS-CoV-2 capturing and isolation

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      Author(s)
      Özkul, Gökçe
      Kehribar, Ebru Şahin
      Ahan, Recep Erdem
      Köksaldı, İlkay Çisil
      Özkul, A.
      Dinç, B.
      Aydoğan, S.
      Şeker, Urartu Özgür Şafak
      Date
      2022-09-13
      Source Title
      Advanced Materials Interfaces
      Print ISSN
      2196-7350
      Electronic ISSN
      2196-7350
      Publisher
      John Wiley & Sons, Ltd
      Volume
      9
      Issue
      30
      Pages
      1 - 11
      Language
      English
      Type
      Article
      Item Usage Stats
      21
      views
      11
      downloads
      Abstract
      The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is continuously infecting people all around the world since its outbreak in 2019. Studies for numerous infection detection strategies are continuing. The sensitivity of detection methods is crucial to separate people with mild infections from people who are asymptomatic. In this sense, a strategy that would help to capture and isolate the SARS-CoV-2 virus prior to tests can be effective and beneficial. To this extent, genetically engineered biomaterials grounding from the biofilm protein of Escherichia coli are beneficial due to their robustness and adaptability to various application areas. Through functionalizing the E. coli biofilm protein, diverse properties can be attained such as enzyme display, nanoparticle production, and medical implant structures. Here, E. coli species are employed to express major curli protein CsgA and Griffithsin (GRFT) as fusion proteins, through a complex formation using SpyTag and SpyCatcher domains. In this study, a complex system with a CsgA scaffold harboring the affinity of GRFT against Spike protein to capture and isolate SARS-CoV-2 virus is successfully developed. It is shown that the hybrid recombinant protein can dramatically increase the sensitivity of currently available lateral flow assays for Sars-CoV-2 diagnostics.
      Keywords
      Genetically engineered biomaterials
      SARS-CoV-2
      Virus capturing
      Concentrating
      Permalink
      http://hdl.handle.net/11693/111241
      Published Version (Please cite this version)
      https://dx.doi.org/10.1002/admi.202201126
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      • Institute of Materials Science and Nanotechnology (UNAM) 2256
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