A genetically engineered biofilm material for SARS-CoV-2 capturing and isolation

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buir.contributor.authorÖzkul, Gökçe
buir.contributor.authorKehribar, Ebru Şahin
buir.contributor.authorAhan, Recep Erdem
buir.contributor.authorKöksaldı, İlkay Çisil
buir.contributor.authorŞeker, Urartu Özgür Şafak
buir.contributor.orcidÖzkul, Gökçe|0000-0003-2592-2899
buir.contributor.orcidKehribar, Ebru Şahin|0000-0003-2951-4390
buir.contributor.orcidAhan, Recep Erdem|0000-0002-6061-9062
buir.contributor.orcidKöksaldı, İlkay Çisil|0000-0002-3121-4939
buir.contributor.orcidŞeker, Urartu Özgür Şafak|0000-0002-5272-1876
dc.citation.epage11en_US
dc.citation.issueNumber30en_US
dc.citation.spage1en_US
dc.citation.volumeNumber9en_US
dc.contributor.authorÖzkul, Gökçe
dc.contributor.authorKehribar, Ebru Şahin
dc.contributor.authorAhan, Recep Erdem
dc.contributor.authorKöksaldı, İlkay Çisil
dc.contributor.authorÖzkul, A.
dc.contributor.authorDinç, B.
dc.contributor.authorAydoğan, S.
dc.contributor.authorŞeker, Urartu Özgür Şafak
dc.date.accessioned2023-02-14T09:00:09Z
dc.date.available2023-02-14T09:00:09Z
dc.date.issued2022-09-13
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractThe novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is continuously infecting people all around the world since its outbreak in 2019. Studies for numerous infection detection strategies are continuing. The sensitivity of detection methods is crucial to separate people with mild infections from people who are asymptomatic. In this sense, a strategy that would help to capture and isolate the SARS-CoV-2 virus prior to tests can be effective and beneficial. To this extent, genetically engineered biomaterials grounding from the biofilm protein of Escherichia coli are beneficial due to their robustness and adaptability to various application areas. Through functionalizing the E. coli biofilm protein, diverse properties can be attained such as enzyme display, nanoparticle production, and medical implant structures. Here, E. coli species are employed to express major curli protein CsgA and Griffithsin (GRFT) as fusion proteins, through a complex formation using SpyTag and SpyCatcher domains. In this study, a complex system with a CsgA scaffold harboring the affinity of GRFT against Spike protein to capture and isolate SARS-CoV-2 virus is successfully developed. It is shown that the hybrid recombinant protein can dramatically increase the sensitivity of currently available lateral flow assays for Sars-CoV-2 diagnostics.en_US
dc.identifier.doi10.1002/admi.202201126en_US
dc.identifier.eissn2196-7350
dc.identifier.issn2196-7350
dc.identifier.urihttp://hdl.handle.net/11693/111241
dc.language.isoEnglishen_US
dc.publisherJohn Wiley & Sons, Ltden_US
dc.relation.isversionofhttps://dx.doi.org/10.1002/admi.202201126en_US
dc.source.titleAdvanced Materials Interfacesen_US
dc.subjectGenetically engineered biomaterialsen_US
dc.subjectSARS-CoV-2en_US
dc.subjectVirus capturingen_US
dc.subjectConcentratingen_US
dc.titleA genetically engineered biofilm material for SARS-CoV-2 capturing and isolationen_US
dc.typeArticleen_US
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Institute of Materials Science and Nanotechnology (UNAM)