Biomineralization with engineered cellular systems

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buir.advisorŞeker, Urartu Özgür Şafak
dc.contributor.authorErgül, Elif
dc.date.accessioned2019-08-27T10:43:33Z
dc.date.available2019-08-27T10:43:33Z
dc.date.copyright2019-08
dc.date.issued2019-08
dc.date.submitted2019-08-26
dc.descriptionCataloged from PDF version of article.en_US
dc.descriptionThesis (Ph.D.): Bilkent University, Department of Materials Science and Nanotechnology, İhsan Doğramacı Bilkent University, 2019.en_US
dc.descriptionIncludes bibliographical references (leaves 89-94).en_US
dc.description.abstractHydroxyapatite (HAP) is the final product of bone biomineralization process and HAP formation is controlled by proteins, enzymes and small molecules secreted to extracellular matrix (ECM). Among these molecules, alkaline phosphatase (ALP) leads formation of HAP crystals and noncollagenous proteins control crystal nucleation and growth, and inhibit crystal formation. Osteocalcin (OCN) and osteopontin (OPN), are the most abundant noncollagenous proteins in ECM, which controls mineralization events. In this study, effect of OCN and OPN on HAP crystal formation was studied in order to achieve controlled crystal growth. In vitro biomineralization assays were conducted to understand the effect of OCN and OPN on the crystal structure of as formed minerals. While OCN decreases crystal growth rate and inhibit mineralization, which leads to more uniform crystal formation, OPN provides faster mineral formation with reduced Ca/P ratio. Moreover, a mammalian engineered cell line was constructed to achieve expression of bone extracellular matrix (ECM) proteins. For this purpose, genetic cassettes were produced to express OCN and OPN proteins, which are the most common non-collagen proteins that control bone mineral formation. By this way, production of bone type minerals with controlled size, shape and Ca/P ratio can be possible. Our system provides a truly biomimetic approach to HAP formation compared to chemical synthesis methods in literature. We believe our current findings will lead to innovative approaches for bone biomineralization in regenerative medicine and bone tissue engineering.en_US
dc.description.provenanceSubmitted by Betül Özen (ozen@bilkent.edu.tr) on 2019-08-27T10:43:32Z No. of bitstreams: 1 THESIS_ELİF ERGÜL.pdf: 7121787 bytes, checksum: f7defa2a97590da3c8a5e37fa06da4f4 (MD5)en
dc.description.provenanceMade available in DSpace on 2019-08-27T10:43:33Z (GMT). No. of bitstreams: 1 THESIS_ELİF ERGÜL.pdf: 7121787 bytes, checksum: f7defa2a97590da3c8a5e37fa06da4f4 (MD5) Previous issue date: 2019-08en
dc.description.statementofresponsibilityby Elif Ergülen_US
dc.format.extentxx, 124 leaves : illustrations (some color), charts (some color) ; 30 cm.en_US
dc.identifier.itemidB108427
dc.identifier.urihttp://hdl.handle.net/11693/52376
dc.language.isoEnglishen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectOsteocalcinen_US
dc.subjectOsteopontinen_US
dc.subjectALP enzymatic activityen_US
dc.subjectBiomineralizationen_US
dc.subjectGenetically modified cellsen_US
dc.titleBiomineralization with engineered cellular systemsen_US
dc.title.alternativeGenetiği değiştirilmiş hücreler ile biyomineralizasyonun sağlanmasıen_US
dc.typeThesisen_US
thesis.degree.disciplineMaterials Science and Nanotechnology
thesis.degree.grantorBilkent University
thesis.degree.levelDoctoral
thesis.degree.namePh.D. (Doctor of Philosophy)

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