Evaluation of ATAD2 as a potential target in hepatocellular carcinoma

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buir.contributor.authorGürsoy Yüzügüllü, Özge
buir.contributor.authorYüzügüllü, Haluk
buir.contributor.orcidGürsoy Yüzügüllü, Özge|0000-0002-3386-3182
buir.contributor.orcidYüzügüllü, Haluk|0000-0002-1039-2998
dc.citation.epage1369en_US
dc.citation.issueNumber52en_US
dc.citation.spage1357en_US
dc.contributor.authorGürsoy Yüzügüllü, Özge
dc.contributor.authorEkin, U.
dc.contributor.authorÖzen, C.
dc.contributor.authorKorhan, P.
dc.contributor.authorBağırsakcı, E.
dc.contributor.authorYılmaz, F.
dc.contributor.authorUzuner, H.
dc.contributor.authorAlotaibi, H.
dc.contributor.authorKırmızıbayrak, P. B.
dc.contributor.authorAtabey, N.
dc.contributor.authorKarakülah, G.
dc.contributor.authorÖztürk, M.
dc.contributor.authorYüzügüllü, Haluk
dc.date.accessioned2022-02-04T08:29:48Z
dc.date.available2022-02-04T08:29:48Z
dc.date.issued2021-11-05
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractPurpose Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide with lack of effec-tive systemic chemotherapy. In this study, we aimed to evaluate the value of ATPase family AAA domain-containing protein 2 (ATAD2) as a biomarker and potential therapeutic target for HCC.Methods The expression of ATAD2 was tested in different HCC patient cohorts by immunohistochemistry and comparative transcriptional analysis. The co-expression of ATAD2 and proliferation markers was compared during liver regeneration and malignancy with different bioinformatics tools. The cellular effects of ATAD2 inactivation in liver malignancy was tested on cell cycle, apoptosis, and colony formation ability as well as tumor formation using RNA interference. The genes affected by ATAD2 inactivation in three different HCC cell lines were identified by global gene expression profiling and bioinformatics tools.Results ATAD2 overexpression is closely correlated with HCC tumor stage. There was gradual increase from dysplasia, well-differentiated and poorly-differentiated HCC, respectively. We also observed transient upregulation of ATAD2 expres-sion during rat liver regeneration in parallel to changes in Ki-67 expression. ATAD2 knockdown resulted in apoptosis and decreased cell survival in vitro and decreased tumor formation in some HCC cell lines. However, three other HCC cell lines tested were not affected. Similarly, gene expression response to ATAD2 inactivation in different HCC cell lines was highly heterogeneous.Conclusions ATAD2 is a potential proliferation marker for liver regeneration and HCC. It may also serve as a therapeutic target despite heterogeneous response of malignant cells.en_US
dc.description.provenanceSubmitted by Bilge Kat (bilgekat@bilkent.edu.tr) on 2022-02-04T08:29:48Z No. of bitstreams: 1 Evaluation_of_ATAD2_as_a_potential_target_in_hepatocellular_carcinoma.pdf: 2581104 bytes, checksum: b27951bdb891e3ed7144000cdbe680d3 (MD5)en
dc.description.provenanceMade available in DSpace on 2022-02-04T08:29:48Z (GMT). No. of bitstreams: 1 Evaluation_of_ATAD2_as_a_potential_target_in_hepatocellular_carcinoma.pdf: 2581104 bytes, checksum: b27951bdb891e3ed7144000cdbe680d3 (MD5) Previous issue date: 5-11-21en
dc.identifier.doi10.1007/s12029-021-00732-9en_US
dc.identifier.eissn1941-6636
dc.identifier.issn1941-6628
dc.identifier.urihttp://hdl.handle.net/11693/77036
dc.language.isoEnglishen_US
dc.publisherSpringeren_US
dc.relation.isversionofhttps://doi.org/10.1007/s12029-021-00732-9en_US
dc.source.titleJournal of Gastrointestinal Canceren_US
dc.subjectLiver canceren_US
dc.subjectATAD2en_US
dc.subjectProliferationen_US
dc.subjectApoptosisen_US
dc.subjectGene expression profilesen_US
dc.subjectGene knock downen_US
dc.titleEvaluation of ATAD2 as a potential target in hepatocellular carcinomaen_US
dc.typeReviewen_US

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