Differential p21 expression after ionizing and UVC radiation in EBV-transformed lymphoblastoid cells

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dc.citation.epage10en_US
dc.citation.issueNumber2en_US
dc.citation.spage1en_US
dc.contributor.authorMoyret-Lalle, C.en_US
dc.contributor.authorLalle P.en_US
dc.contributor.authorPedeux, R.en_US
dc.contributor.authorGuillot, C.en_US
dc.contributor.authorMartel, S.en_US
dc.contributor.authorMagaud J.-P.en_US
dc.contributor.authorPuisieux, A.en_US
dc.contributor.authorOzturk, M.en_US
dc.date.accessioned2016-02-08T10:36:30Z
dc.date.available2016-02-08T10:36:30Z
dc.date.issued2000en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractResponses to DNA-damaging agents appear to be coordinated by p53 through transcriptional activation of critical target genes. Among them, p21WAF1 encodes a protein preventing cells from entering S phase. It is not clear whether p53-mediated response varies depending on the type of DNA damage. Here, we have decided to compare the p53-mediated response of EBV-transformed lymphoblasts to ionizing radiation and UVC irradiation. We have shown that these cells respond to ionizing radiation by a cell cycle arrest as expected. Surprisingly they failed to do so after UVC treatment. Accordingly there was no significant induction of p21 protein in UVC exposed cells despite p53 accumulation. Using isogenic EBV-transformed lymphoblastoid cells expressing E6 protein of HPV18, we have demonstrated that there was no evidence of p53-dependent cell cycle arrest after UVC irradiation. These observations suggest that the p53-mediated response to UVC, in contrast to ionizing radiation, was compromised in EBV-transformed cells and might be cell type-dependent.en_US
dc.identifier.issn12928933
dc.identifier.urihttp://hdl.handle.net/11693/24939
dc.language.isoEnglishen_US
dc.source.titleElectronic Journal of Oncologyen_US
dc.subjectprotein E6en_US
dc.subjectprotein MDM2en_US
dc.subjectprotein p21en_US
dc.subjectprotein p53en_US
dc.subjectarticleen_US
dc.subjectcell cycleen_US
dc.subjectcell cycle S phaseen_US
dc.subjectcell growthen_US
dc.subjectcell transformationen_US
dc.subjectcellular distributionen_US
dc.subjectcontrolled studyen_US
dc.subjectDNA damageen_US
dc.subjectEpstein Barr virusen_US
dc.subjectgene targetingen_US
dc.subjectgenetic transcriptionen_US
dc.subjectgrowth inhibitionen_US
dc.subjecthumanen_US
dc.subjecthuman cellen_US
dc.subjectionizing radiationen_US
dc.subjectlymphoblastoid cellen_US
dc.subjectprotein expressionen_US
dc.subjectprotein functionen_US
dc.subjectprotein inductionen_US
dc.subjectprotein localizationen_US
dc.subjectultraviolet C radiationen_US
dc.titleDifferential p21 expression after ionizing and UVC radiation in EBV-transformed lymphoblastoid cellsen_US
dc.typeArticleen_US

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