Suppression of aquaporin-4 by antisense oligonucleotides reduces brain edema in experimental traumatic brain injury

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buir.contributor.orcidKarlı Oğuz, Hatice Kader|0000-0002-3385-4665
dc.citation.epage922en_US
dc.citation.issueNumber6en_US
dc.citation.spage916en_US
dc.citation.volumeNumber32en_US
dc.contributor.authorHekimoğlu, Mehdi
dc.contributor.authorLule, Sevda
dc.contributor.authorÖzer, Hıdır
dc.contributor.authorÇakır-Aktas, Canan
dc.contributor.authorMut, Melike
dc.contributor.authorKarlı Oğuz, Hatice Kader
dc.date.accessioned2023-02-28T13:24:25Z
dc.date.available2023-02-28T13:24:25Z
dc.date.issued2022-03-08
dc.departmentAysel Sabuncu Brain Research Center (BAM)en_US
dc.description.abstractAIM: To investigate the suppression of aquaporin-4 (AQP4) synthesis through intracerebroventricular (i.c.v.) injection of antisense oligonucleotide after focal cortical contusion injury in mice. MATERIAL and METHODS: This study used 12-week-old female Swiss albino mice (weight, 20–25 g) to create a focal cortical contusion model by the weight-drop method (35 g blunt weight, 70 cm height) onto the parietal cortex after craniectomy. The sham group underwent craniectomy without trauma. In the control group, weight was dropped onto the parietal cortex immediately after i.c.v. injection of Dulbecco’s Modified Eagle Medium after craniectomy. In addition, 1 nM of aquaporin-4 (AQP4) antisense oligonucleotide (ASO) was injected via the i.c.v. route immediately after trauma (0 hour) and 4 hours after trauma. All animals underwent magnetic resonance (MR) imaging and were sacrificed at 24 hours. The brain–water content was determined using the wet/dry weight method. RESULTS: In the sham group, the average percentage of the brain–water content was 77.75% compared with the control group with 79.87%, and the difference was significant (p=0.017). The average was 78.81% and significantly reduced in the therapy group compared with the control group (p=0.026) at 0 hour. In the 4-hour treatment group, the average of 79.11% was not significant (p=0.39). MR imaging findings also showed a substantial reduction in brain edema in the 0-h treatment group. However, the 4-h treatment results, when compared with the control trauma group, did not show a significant difference. CONCLUSION: This study demonstrated that AQP4-ASO therapy, when administered early after diffuse traumatic brain injury, leads to a significant reduction in brain edema. © 2022, The Korean Society of Clothing and Textiles. All rights reserved.en_US
dc.description.provenanceSubmitted by Zeliha Bucak Çelik (zeliha.celik@bilkent.edu.tr) on 2023-02-28T13:24:25Z No. of bitstreams: 1 Suppression_of_aquaporin-4_by_antisense_oligonucleotides_reduces_brain_edema_in_experimental_traumatic_brain_injury.pdf: 879054 bytes, checksum: f6c88c2cb0c01e9f478a00a68de5636d (MD5)en
dc.description.provenanceMade available in DSpace on 2023-02-28T13:24:25Z (GMT). No. of bitstreams: 1 Suppression_of_aquaporin-4_by_antisense_oligonucleotides_reduces_brain_edema_in_experimental_traumatic_brain_injury.pdf: 879054 bytes, checksum: f6c88c2cb0c01e9f478a00a68de5636d (MD5) Previous issue date: 2022-03-08en
dc.identifier.doi10.5137/1019-5149.JTN.36355-21.3en_US
dc.identifier.issn1019-5149
dc.identifier.urihttp://hdl.handle.net/11693/111945
dc.language.isoEnglishen_US
dc.publisherTurkish Neurosurgical Societyen_US
dc.relation.isversionofhttps://dx.doi.org/10.5137/1019-5149.JTN.36355-21.3en_US
dc.source.titleTurkish Neurosurgeryen_US
dc.subjectAntisense oligonucleotideen_US
dc.subjectAquaporin-4en_US
dc.subjectBrain edemaen_US
dc.subjectMiceen_US
dc.subjectMrien_US
dc.subjectTraumatic brain injuryen_US
dc.titleSuppression of aquaporin-4 by antisense oligonucleotides reduces brain edema in experimental traumatic brain injuryen_US
dc.typeArticleen_US

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