Examination of fabrication conditions of acrylate-based hydrogel formulations for doxorubicin release and efficacy test for hepatocellular carcinoma cell

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dc.citation.epage678en_US
dc.citation.issueNumber7en_US
dc.citation.spage657en_US
dc.citation.volumeNumber25en_US
dc.contributor.authorBayramoglu, G.en_US
dc.contributor.authorGozen, Damlaen_US
dc.contributor.authorErsoy, G.en_US
dc.contributor.authorOzalp, V. C.en_US
dc.contributor.authorAkcali, K. C.en_US
dc.contributor.authorArica, M. Y.en_US
dc.date.accessioned2016-02-08T10:54:43Z
dc.date.available2016-02-08T10:54:43Z
dc.date.issued2014en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractThe objective of the present study was to develop 2-hydroxypropyl methacrylate-co-polyethylene methacrylate [p(HPMA-co-PEG-MEMA)] hydrogels that are able to efficiently entrap doxorubicin for the application of loco-regional control of the cancer disease. Systemic chemotherapy provides low clinical benefit while localized chemotherapy might provide a therapeutic advantage. In this study, effects of hydrogel properties such as PEG chains length, cross-linking density, biocompatibility, drug loading efficiency, and drug release kinetics were evaluated in vitro for targeted and controlled drug delivery. In addition, the characterization of the hydrogel formulations was conducted with swelling experiments, permeability tests, Fourier transform infrared, SEM, and contact angle studies. In these drug-hydrogel systems, doxorubicin contains amine group that can be expected a strong Lewis acid-base interaction between drug and polar groups of PEG chains, thus the drug was released in a timely fashion with an electrostatic interaction mechanism. It was observed that doxorubicin release from the hydrogel formulations decreased when the density of cross-linking, and drug/polymer ratio were increased while an increase in the PEG chains length of the macro-monomer (i.e. PEG-MEMA) in the hydrogel system was associated with an increase in water content and doxorubicin release. The biocompatibility of the hydrogel formulations has been investigated using two measures: cytotoxicity test (using lactate dehydrogenase assay) and major serum proteins adsorption studies. Antitumor activity of the released doxorubicin was assessed using a human SNU398 human hepatocellular carcinoma cell line. It was observed that doxorubicin released from all of our hydrogel formulations which remained biologically active and had the capability to kill the tested cancer cells.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:54:43Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2014en
dc.identifier.doi10.1080/09205063.2014.890920en_US
dc.identifier.issn0920-5063
dc.identifier.urihttp://hdl.handle.net/11693/26080
dc.language.isoEnglishen_US
dc.publisherTaylor and Francis.en_US
dc.relation.isversionofhttp://dx.doi.org/10.1080/09205063.2014.890920en_US
dc.source.titleJournal of Biomaterials Science, Polymer Editionen_US
dc.subjectBiocompatibilityen_US
dc.subjectBiomedical applicationsen_US
dc.subjectDoxorubicinen_US
dc.subjectDrug deliveryen_US
dc.subjectHepatocellular carcinoma cellen_US
dc.subjectHydrogelsen_US
dc.titleExamination of fabrication conditions of acrylate-based hydrogel formulations for doxorubicin release and efficacy test for hepatocellular carcinoma cellen_US
dc.typeArticleen_US

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