Evaporation-induced biomolecule detection on versatile superhydrophilic patterned surfaces: glucose and DNA assay

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buir.contributor.authorBeyazkilic, Pınar
buir.contributor.authorSaateh, Abtin
buir.contributor.authorBayındır, Mehmet
buir.contributor.authorElbuken, Çaglar
dc.citation.epage13509en_US
dc.citation.issueNumber10en_US
dc.citation.spage13503en_US
dc.citation.volumeNumber3en_US
dc.contributor.authorBeyazkilic, Pınaren_US
dc.contributor.authorSaateh, Abtinen_US
dc.contributor.authorBayındır, Mehmeten_US
dc.contributor.authorElbuken, Çaglaren_US
dc.date.accessioned2019-02-21T16:02:25Z
dc.date.available2019-02-21T16:02:25Z
dc.date.issued2018en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.description.abstractWe introduce a droplet-based biomolecular detection platform using robust, versatile, and low-cost superhydrophilic patterned superhydrophobic surfaces. Benefitting from confinement and evaporation-induced shrinkage of droplets on wetted patterns, we show enrichment-based biomolecular detection using very low sample volumes. First, we developed a glucose assay using fluorescent polydopamine (PDA) based on enhancement of PDA emission by hydrogen peroxide (H2O2) produced in enzyme-mediated glucose oxidation reaction. Incubation in evaporating droplets resulted in brighter fluorescence compared to that in bulk solutions. Droplet assay was highly sensitive toward increasing glucose concentration while that in milliliter-volume solutions resulted in no fluorescence enhancement at similar time scales. This is due to droplet evaporation that increased the reaction rate by causing enrichment of PDA and glucose/glucose oxidase as well as increased concentration of H2O2 generated in shrinking droplet. Second, we chemically functionalized wetted patterns with single-stranded DNA and developed fluorescence-based DNA detection to demonstrate the adaptability of the patterned surfaces for a different class of assay. We achieved detection of glucose and DNA with concentration down to 130 μM and 200 fM, respectively. Patterned superhydrophobic surfaces with their simple production, sensitive response, and versatility present potential for bioanalysis from low sample volumes. Copyright
dc.description.provenanceMade available in DSpace on 2019-02-21T16:02:25Z (GMT). No. of bitstreams: 1 Bilkent-research-paper.pdf: 222869 bytes, checksum: 842af2b9bd649e7f548593affdbafbb3 (MD5) Previous issue date: 2018en
dc.description.sponsorshipP.B. was supported by TÜBITAK-BİDEḂ graduate fellowship. This study is partly supported by TÜBITAK̇ under the Project no. 111T696.
dc.identifier.doi10.1021/acsomega.8b00389
dc.identifier.issn2470-1343
dc.identifier.urihttp://hdl.handle.net/11693/50001
dc.language.isoEnglish
dc.publisherAmerican Chemical Society
dc.relation.isversionofhttps://doi.org/10.1021/acsomega.8b00389
dc.relation.project111T696
dc.rightsinfo:eu-repo/semantics/openAccess
dc.source.titleACS Omegaen_US
dc.titleEvaporation-induced biomolecule detection on versatile superhydrophilic patterned surfaces: glucose and DNA assayen_US
dc.typeArticleen_US

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