Distinct regulation of tonsillar immune response in virus infection

dc.citation.epage667en_US
dc.citation.issueNumber5en_US
dc.citation.spage658en_US
dc.citation.volumeNumber69en_US
dc.contributor.authorJartti, T.en_US
dc.contributor.authorPalomares, O.en_US
dc.contributor.authorWaris, M.en_US
dc.contributor.authorTastan, O.en_US
dc.contributor.authorNieminen, R.en_US
dc.contributor.authorPuhakka, T.en_US
dc.contributor.authorRückert, B.en_US
dc.contributor.authorAab, A.en_US
dc.contributor.authorVuorinen, T.en_US
dc.contributor.authorAllander, T.en_US
dc.contributor.authorVahlberg, T.en_US
dc.contributor.authorRuuskanen, O.en_US
dc.contributor.authorAkdis, M.en_US
dc.contributor.authorAkdis, C. A.en_US
dc.date.accessioned2016-02-08T11:01:53Z
dc.date.available2016-02-08T11:01:53Z
dc.date.issued2014en_US
dc.departmentDepartment of Computer Engineeringen_US
dc.description.abstractBackground: The relationships between tonsillar immune responses, and viral infection and allergy are incompletely known. Objective To study intratonsillar/nasopharyngeal virus detections and in vivo expressions of T-cell- and innate immune response-specific cytokines, transcription factors, and type I/II/III interferons in human tonsils. Methods: Palatine tonsil samples were obtained from 143 elective tonsillectomy patients. Adenovirus, bocavirus-1, coronavirus, enteroviruses, influenza virus, metapneumovirus, parainfluenza virus, rhinovirus, and respiratory syncytial virus were detected using PCR. The mRNA expression levels of IFN-α, IFN-β, IFN-γ, IL-10, IL-13, IL-17, IL-28, IL-29, IL-37, TGF-β, FOXP3, GATA3, RORC2, and Tbet were directly analyzed by quantitative RT-PCR. Results Fifty percentage of subjects reported allergy, 59% had ≥1 nasopharyngeal viruses, and 24% had ≥1 intratonsillar viruses. Tonsillar virus detection showed a strong negative association with age; especially rhinovirus or parainfluenza virus detection showed positive association with IFN-γ and Tbet expressions. IL-37 expression was positively associated with atopic dermatitis, whereas IFN-α, IL-13, IL-28, and Tbet expressions were negatively associated with allergic diseases. Network analyses demonstrated strongly polarized clusters of immune regulatory (IL-10, IL-17, TGF-β, FOXP3, GATA3, RORC2, Tbet) and antiviral (IFN-α, IFN-β, IL-28, IL-29) genes. These two clusters became more distinctive in the presence of viral infection or allergy. A negative correlation between antiviral cytokines and IL-10, IL-17, IL-37, FOXP3, and RORC2 was observed only in the presence of viruses, and interestingly, IL-13 strongly correlated with antiviral cytokines. Conclusions: Tonsillar cytokine expression is closely related to existing viral infections, age, and allergic illnesses and shows distinct clusters between antiviral and immune regulatory genes. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T11:01:53Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2014en
dc.identifier.doi10.1111/all.12396en_US
dc.identifier.issn0105-4538en_US
dc.identifier.urihttp://hdl.handle.net/11693/26584en_US
dc.language.isoEnglishen_US
dc.publisherWiley-Blackwell Publishing Ltd.en_US
dc.relation.isversionofhttp://dx.doi.org/10.1111/all.12396en_US
dc.source.titleAllergyen_US
dc.subjectAllergyen_US
dc.subjectCytokineen_US
dc.subjectT cellen_US
dc.subjectTonsilen_US
dc.subjectVirusen_US
dc.subjectAlpha interferonen_US
dc.subjectBeta interferonen_US
dc.subjectGamma interferonen_US
dc.subjectInterferonen_US
dc.subjectTranscription factoren_US
dc.subjectBocavirusen_US
dc.subjectMetapneumovirusen_US
dc.subjectNasopharynxen_US
dc.subjectNonhumanen_US
dc.subjectPalatine tonsilen_US
dc.subjectParainfluenza virusen_US
dc.subjectPolymerase chain reaction systemen_US
dc.subjectPriority journalen_US
dc.subjectProtein expressionen_US
dc.subjectRegulatory mechanismen_US
dc.subjectRespiratory syncytial pneumovirusen_US
dc.subjectRhinovirusen_US
dc.subjectT lymphocyteen_US
dc.subjectTonsillectomyen_US
dc.subjectVirus detectionen_US
dc.subjectVirus infectionen_US
dc.subjectCluster analysisen_US
dc.subjectGene expression profilingen_US
dc.subjectGene expression regulationen_US
dc.subjectGene regulatory networken_US
dc.subjectGeneticsen_US
dc.subjectImmunologyen_US
dc.subjectMetabolismen_US
dc.subjectPalatine tonsilen_US
dc.subjectVirologyen_US
dc.subjectVirus infectionen_US
dc.subjectYoung adulten_US
dc.subjectAdolescenten_US
dc.subjectGene expression regulationen_US
dc.subjectGene regulatory networksen_US
dc.titleDistinct regulation of tonsillar immune response in virus infectionen_US
dc.typeArticleen_US

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