Type I IFN-related NETosis in ataxia telangiectasia and Artemis deficiency

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buir.contributor.authorKara-Eroğlu, Fehime
buir.contributor.authorKahraman, Tamer
buir.contributor.authorGürsel, İhsan
dc.citation.epage257en_US
dc.citation.issueNumber1en_US
dc.citation.spage246en_US
dc.citation.volumeNumber142en_US
dc.contributor.authorGül, E.en_US
dc.contributor.authorSayar, E. H.en_US
dc.contributor.authorGüngör, B.en_US
dc.contributor.authorKara-Eroğlu, Fehimeen_US
dc.contributor.authorSürücü, N.en_US
dc.contributor.authorKeleş, S.en_US
dc.contributor.authorGüner, S. N.en_US
dc.contributor.authorFındık, S.en_US
dc.contributor.authorAlpdündar, E.en_US
dc.contributor.authorAyanoğlu, I. C.en_US
dc.contributor.authorKayaoğlu, B.en_US
dc.contributor.authorGeçkin, B. N.en_US
dc.contributor.authorŞanlı, H. A.en_US
dc.contributor.authorKahraman, Tameren_US
dc.contributor.authorYakıcıer, C.en_US
dc.contributor.authorMüftüoğlu, M.en_US
dc.contributor.authorOğuz, B.en_US
dc.contributor.authorAyvaz, D. N. Ç.en_US
dc.contributor.authorGürsel, İhsanen_US
dc.contributor.authorÖzen, S.en_US
dc.contributor.authorReisli, İ.en_US
dc.contributor.authorGürsel, M.en_US
dc.date.accessioned2019-02-21T16:01:38Zen_US
dc.date.available2019-02-21T16:01:38Zen_US
dc.date.issued2018en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractBackground: Pathological inflammatory syndromes of unknown etiology are commonly observed in ataxia telangiectasia (AT) and Artemis deficiency. Similar inflammatory manifestations also exist in patients with STING-associated vasculopathy in infancy (SAVI). Objective: We sought to test the hypothesis that the inflammation-associated manifestations observed in patients with AT and Artemis deficiency stem from increased type I IFN signature leading to neutrophil-mediated pathological damage. Methods: Cytokine/protein signatures were determined by ELISA, cytometric bead array, or quantitative PCR. Stat1 phosphorylation levels were determined by flow cytometry. DNA species accumulating in the cytosol of patients' cells were quantified microscopically and flow cytometrically. Propensity of isolated polymorhonuclear granulocytes to form neutrophil extracellular traps (NETs) was determined using fluorescence microscopy and picogreen assay. Neutrophil reactive oxygen species levels and mitochondrial stress were assayed using fluorogenic probes, microscopy, and flow cytometry. Results: Type I and III IFN signatures were elevated in plasma and peripheral blood cells of patients with AT, Artemis deficiency, and SAVI. Chronic IFN production stemmed from the accumulation of DNA in the cytoplasm of AT and Artemis-deficient cells. Neutrophils isolated from patients spontaneously produced NETs and displayed indicators of oxidative and mitochondrial stress, supportive of their NETotic tendencies. A similar phenomenon was also observed in neutrophils from healthy controls exposed to patient plasma samples or exogeneous IFN-α. Conclusions: Type I IFN-mediated neutrophil activation and NET formation may contribute to inflammatory manifestations observed in patients with AT, Artemis deficiency, and SAVI. Thus, neutrophils represent a promising target to manage inflammatory syndromes in diseases with active type I IFN signature.en_US
dc.identifier.doi10.1016/j.jaci.2017.10.030en_US
dc.identifier.doi10.1016/j.jaci.2017.10.030en_US
dc.identifier.eissn1097-6825en_US
dc.identifier.issn0091-6749en_US
dc.identifier.urihttp://hdl.handle.net/11693/49891en_US
dc.language.isoEnglishen_US
dc.publisherMosbyen_US
dc.relation.isversionofhttps://doi.org/10.1016/j.jaci.2017.10.030en_US
dc.source.titleJournal of Allergy and Clinical Immunologyen_US
dc.subjectArtemis deficiencyen_US
dc.subjectAtaxia telangiectasiaen_US
dc.subjectAutoinflammationen_US
dc.subjectInterferonopathyen_US
dc.subjectNETosisen_US
dc.subjectNeutrophil extracellular trapsen_US
dc.subjectPrimary immunodeficienciesen_US
dc.subjectType I IFNen_US
dc.titleType I IFN-related NETosis in ataxia telangiectasia and Artemis deficiencyen_US
dc.typeArticleen_US

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