Chemical and topographical modification of PHBV surface to promote osteoblast alignment and confinement

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buir.contributor.authorAydınlı, Atilla
dc.citation.epage1010en_US
dc.citation.issueNumber4en_US
dc.citation.spage1001en_US
dc.citation.volumeNumber85Aen_US
dc.contributor.authorKenar, H.en_US
dc.contributor.authorKocabas, A.en_US
dc.contributor.authorAydınlı, Atillaen_US
dc.contributor.authorHasirci, V.en_US
dc.date.accessioned2016-02-08T10:08:46Z
dc.date.available2016-02-08T10:08:46Z
dc.date.issued2008en_US
dc.departmentDepartment of Physicsen_US
dc.description.abstractProper cell attachment and distribution, and thus stronger association in vivo between a bone implant and native tissue will improve the success of the implant. In this study, the aim was to achieve promotion of attachment and uniform distribution of rat mesenchymal stem cell-derived osteoblasts by introducing chemical and topographical cues on poly(3-hydroxybutyrate-co-3- hydroxyvalerate) (PHBV) film surfaces. As the chemical cues, either alkaline phosphatase was covalently immobilized on the film surface to induce deposition of calcium phosphate minerals or fibrinogen was adsorbed to improve cell adhesion. Microgrooves and micropits were introduced on the film surface by negative replication of micropatterned Si wafers. Both chemical cues improved cell attachment and even distribution on the PHBV films, but Fb was more effective especially when combined with the micropatterns. Cell alignment (<10° deviation angle) parallel to chemically modified microgrooves (1, 3, or 8 μm groove width) and on 10 μm-thick Fb lines printed on the unpatterned films was achieved. The cells on unpatterned and 5 μm-deep micropitted films were distributed and oriented randomly. Results of this study proved that microtopographies on PHBV can improve osseointegration when combined with chemical cues, and that microgrooves and cell adhesive protein lines on PHBV can guide selective osteoblast adhesion and alignment.en_US
dc.identifier.doi10.1002/jbm.a.31638en_US
dc.identifier.issn1549-3296
dc.identifier.urihttp://hdl.handle.net/11693/23088
dc.language.isoEnglishen_US
dc.publisherJohn Wiley & Sons, Inc.en_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/jbm.a.31638en_US
dc.source.titleJournal of Biomedical Materials Research - Part Aen_US
dc.subjectBone tissue engineeringen_US
dc.subjectMicropatterned filmsen_US
dc.subjectOsteoblastsen_US
dc.subjectPHBVen_US
dc.subjectPhotolithographyen_US
dc.subjectBoneen_US
dc.subjectCell cultureen_US
dc.subjectImplants (surgical)en_US
dc.subjectPhotolithographyen_US
dc.subjectSilicon wafersen_US
dc.subjectCell attachmenten_US
dc.subjectFilm surfaceen_US
dc.subjectMicropatterned filmsen_US
dc.subjectOsteoblastsen_US
dc.subjectAlkaline phosphataseen_US
dc.subjectCalcium phosphateen_US
dc.subjectFibrinogenen_US
dc.subjectPoly (3 hydroxybutyric acid)en_US
dc.subjectSiliconen_US
dc.subjectValeric acid derivativeen_US
dc.subjectAnimal cellen_US
dc.subjectBone regenerationen_US
dc.subjectCell adhesionen_US
dc.subjectCellular distributionen_US
dc.subjectChemical cueen_US
dc.subjectChemical modificationen_US
dc.subjectControlled studyen_US
dc.subjectMesenchyme cellen_US
dc.subjectNonhumanen_US
dc.subjectOsteoblasten_US
dc.subjectRaten_US
dc.subjectSurface propertyen_US
dc.subjectTissue engineeringen_US
dc.subjectTopographyen_US
dc.subjectAcridine orangeen_US
dc.subjectAnimalsen_US
dc.subjectCell adhesionen_US
dc.subjectCell counten_US
dc.subjectCells, cultureden_US
dc.subjectOsteoblastsen_US
dc.subjectPolyestersen_US
dc.subjectPolystyrenesen_US
dc.subjectRats, Sprague-Dawleyen_US
dc.subjectSurface Propertiesen_US
dc.titleChemical and topographical modification of PHBV surface to promote osteoblast alignment and confinementen_US
dc.typeArticleen_US

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