Early detection and staging of colorectal cancer using a panel of micro RNAs
buir.contributor.author | Güre, Ali O. | |
dc.citation.epage | 168 | en_US |
dc.citation.issueNumber | 7 | en_US |
dc.citation.spage | 162 | en_US |
dc.citation.volumeNumber | 10 | en_US |
dc.contributor.author | Shapira, R. | en_US |
dc.contributor.author | Ilyayev, N. | en_US |
dc.contributor.author | Attali, R. | en_US |
dc.contributor.author | Westrich, G. | en_US |
dc.contributor.author | Halle, D. | en_US |
dc.contributor.author | Speter, C. | en_US |
dc.contributor.author | Stavropoulos, A. V. | en_US |
dc.contributor.author | Roistacher, M. | en_US |
dc.contributor.author | Pavlov, V. | en_US |
dc.contributor.author | Grinbaum, R. | en_US |
dc.contributor.author | Protic, P. | en_US |
dc.contributor.author | Güre, Ali O. | en_US |
dc.contributor.author | Bilchik, A. J. | en_US |
dc.contributor.author | Stojadinovic, A. | en_US |
dc.contributor.author | Mitrani-Rosenbaum, S. | en_US |
dc.contributor.author | Nissan, A. | en_US |
dc.date.accessioned | 2019-02-24T12:27:52Z | |
dc.date.available | 2019-02-24T12:27:52Z | |
dc.date.issued | 2018 | en_US |
dc.department | Department of Molecular Biology and Genetics | en_US |
dc.description.abstract | Purpose: To improve lymph node (LN) staging in patients with colon cancer (CC). The present study describes the selection of CC-specific miRNAs and assesses their utility as a micro metastases detection assay. Methods: 30 miRNAs have been selected from a microarray assay and 16 miRNAs from database mining for their specific upregulation in colon cancer tissues as compared to normal adjacent tissues. Differential expression was validated by RT-qPCR in a larger cohort of samples (n=20) and compared to normal lymphatic tissues (n=6) and normal peripheral blood lymphocytes (PBLs, n=14). The selected miRNA panel was then used for the screening of 84 lymph nodes (LN) obtained from colon cancer patients (n=20) Results: After validation, a panel of 8 miRNAs was found to be significantly upregulated in CC compared to normal adjacent tissues and to normal lymphatic tissues: miR-96, miR-183, miR-194, miR-200a, miR-200b, miR200c, miR-203 and miR-429. A total of 84 LNs were analysed: 12 LN metastases were detected by H&E, 18 by CK staining whereas 32 were detected by the CC-specific miRNA analysis. This represents an increase of 40% in the detection rate. Conclusion: This study demonstrated the ability of a CC-specific 8 miRNA panel in detecting micro metastases in CC patients. | en_US |
dc.description.provenance | Submitted by Onur Emek (onur.emek@bilkent.edu.tr) on 2019-02-24T12:27:52Z No. of bitstreams: 1 Early_Detection_and_Staging_of_Colorectal_Cancer_Using_a_Panel_of.pdf: 547552 bytes, checksum: 39454cf7975d72883d5d67335b8d84ae (MD5) | en |
dc.description.provenance | Made available in DSpace on 2019-02-24T12:27:52Z (GMT). No. of bitstreams: 1 Early_Detection_and_Staging_of_Colorectal_Cancer_Using_a_Panel_of.pdf: 547552 bytes, checksum: 39454cf7975d72883d5d67335b8d84ae (MD5) Previous issue date: 2018 | en |
dc.identifier.doi | 10.4172/1948-5956.1000539 | en_US |
dc.identifier.issn | 1948-5956 | |
dc.identifier.uri | http://hdl.handle.net/11693/50581 | |
dc.language.iso | English | en_US |
dc.publisher | OMICS | en_US |
dc.relation.isversionof | https://doi.org/10.4172/1948-5956.1000539 | en_US |
dc.source.title | Journal of Cancer Science & Therapy | en_US |
dc.subject | Colorectal cancer | en_US |
dc.subject | miRNA | en_US |
dc.subject | Staging | en_US |
dc.subject | Colorectal metastases | en_US |
dc.subject | Tumor markers | en_US |
dc.title | Early detection and staging of colorectal cancer using a panel of micro RNAs | en_US |
dc.type | Article | en_US |
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