Early detection and staging of colorectal cancer using a panel of micro RNAs

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buir.contributor.authorGüre, Ali O.
dc.citation.epage168en_US
dc.citation.issueNumber7en_US
dc.citation.spage162en_US
dc.citation.volumeNumber10en_US
dc.contributor.authorShapira, R.en_US
dc.contributor.authorIlyayev, N.en_US
dc.contributor.authorAttali, R.en_US
dc.contributor.authorWestrich, G.en_US
dc.contributor.authorHalle, D.en_US
dc.contributor.authorSpeter, C.en_US
dc.contributor.authorStavropoulos, A. V.en_US
dc.contributor.authorRoistacher, M.en_US
dc.contributor.authorPavlov, V.en_US
dc.contributor.authorGrinbaum, R.en_US
dc.contributor.authorProtic, P.en_US
dc.contributor.authorGüre, Ali O.en_US
dc.contributor.authorBilchik, A. J.en_US
dc.contributor.authorStojadinovic, A.en_US
dc.contributor.authorMitrani-Rosenbaum, S.en_US
dc.contributor.authorNissan, A.en_US
dc.date.accessioned2019-02-24T12:27:52Z
dc.date.available2019-02-24T12:27:52Z
dc.date.issued2018en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractPurpose: To improve lymph node (LN) staging in patients with colon cancer (CC). The present study describes the selection of CC-specific miRNAs and assesses their utility as a micro metastases detection assay. Methods: 30 miRNAs have been selected from a microarray assay and 16 miRNAs from database mining for their specific upregulation in colon cancer tissues as compared to normal adjacent tissues. Differential expression was validated by RT-qPCR in a larger cohort of samples (n=20) and compared to normal lymphatic tissues (n=6) and normal peripheral blood lymphocytes (PBLs, n=14). The selected miRNA panel was then used for the screening of 84 lymph nodes (LN) obtained from colon cancer patients (n=20) Results: After validation, a panel of 8 miRNAs was found to be significantly upregulated in CC compared to normal adjacent tissues and to normal lymphatic tissues: miR-96, miR-183, miR-194, miR-200a, miR-200b, miR200c, miR-203 and miR-429. A total of 84 LNs were analysed: 12 LN metastases were detected by H&E, 18 by CK staining whereas 32 were detected by the CC-specific miRNA analysis. This represents an increase of 40% in the detection rate. Conclusion: This study demonstrated the ability of a CC-specific 8 miRNA panel in detecting micro metastases in CC patients.en_US
dc.identifier.doi10.4172/1948-5956.1000539en_US
dc.identifier.issn1948-5956
dc.identifier.urihttp://hdl.handle.net/11693/50581
dc.language.isoEnglishen_US
dc.publisherOMICSen_US
dc.relation.isversionofhttps://doi.org/10.4172/1948-5956.1000539en_US
dc.source.titleJournal of Cancer Science & Therapyen_US
dc.subjectColorectal canceren_US
dc.subjectmiRNAen_US
dc.subjectStagingen_US
dc.subjectColorectal metastasesen_US
dc.subjectTumor markersen_US
dc.titleEarly detection and staging of colorectal cancer using a panel of micro RNAsen_US
dc.typeArticleen_US

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