Detection of Calcium-induced morphological changes on RBCs by digital holographic microscopy and blinking optical tweezers

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dc.citation.epage3en_US
dc.citation.spage1en_US
dc.contributor.authorRad, V. F.en_US
dc.contributor.authorTavakkoli, R.en_US
dc.contributor.authorMoradi, Ali-Rezaen_US
dc.contributor.authorAnand, A.en_US
dc.contributor.authorJavidi, B.en_US
dc.coverage.spatialBarcelona, Spainen_US
dc.date.accessioned2018-04-12T11:47:54Z
dc.date.available2018-04-12T11:47:54Z
dc.date.issued2016en_US
dc.departmentDepartment of Physicsen_US
dc.descriptionDate of Conference: 11-15 July 2016en_US
dc.descriptionConference Name: 15th Workshop on Information Optics, WIO 2016en_US
dc.description.abstractCa+2 level in the circulating red blood cells (RBCs) takes part not only in controlling biophysical properties, but also affects the membrane composition, and its morphological and rheological properties. Excessive accumulation of Ca2+ within the cells is associated with a number of important pathological diseases. In this paper, by the use of digital holographic microscopy (DHM), we quantitatively analyzed the volumetric behavior of RBC membrane under influence of excess Calcium ions. DHM in a transmission mode is an effective tool for quantitative visualization of phase objects. By deriving the associated phase changes 3D information on the morphology variation of the cells at arbitrary time scales is obtained. Individual cells are immobilized by the use of optical tweezers and are monitored live with DHM system, while the concentration of Ca2+ ions in the buffer is changed simultaneously. We utilized blinking optical tweezers, by inserting an optical chopper to modulate intensity of the trapping laser beam. Blinking optical tweezers, while keeping the cell trapped during the experiments, ensures of minimizing the photo-damage of trapping laser beam on the cell. Our experimental results are in agreement with previous biological studies and predictions, and experimental observations of living RBCs under Ca2+ influence.en_US
dc.description.provenanceMade available in DSpace on 2018-04-12T11:47:54Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 179475 bytes, checksum: ea0bedeb05ac9ccfb983c327e155f0c2 (MD5) Previous issue date: 2016en
dc.identifier.doi10.1109/WIO.2016.7745588en_US
dc.identifier.urihttp://hdl.handle.net/11693/37683
dc.language.isoEnglishen_US
dc.publisherIEEEen_US
dc.relation.isversionofhttp://dx.doi.org/10.1109/WIO.2016.7745588en_US
dc.source.titleProceedings of the 15th Workshop on Information Optics, WIO 2016en_US
dc.subjectCalciumen_US
dc.subjectCellsen_US
dc.subjectCytologyen_US
dc.subjectHolographyen_US
dc.subjectIonsen_US
dc.subjectLaser beamsen_US
dc.subjectMicroscopic examinationen_US
dc.subjectPhase measurementen_US
dc.subjectBiophysical propertiesen_US
dc.subjectDigital holographic microscopyen_US
dc.subjectMembrane compositionen_US
dc.subjectMorphological changesen_US
dc.subjectPathological diseaseen_US
dc.subjectQuantitative visualizationen_US
dc.subjectRheological propertyen_US
dc.subjectVolumetric behavioren_US
dc.subjectOptical tweezersen_US
dc.titleDetection of Calcium-induced morphological changes on RBCs by digital holographic microscopy and blinking optical tweezersen_US
dc.typeConference Paperen_US

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