Global miRNA expression of bone marrow mesenchymal stem/stromal cells derived from Fanconi anemia patients

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buir.contributor.authorKeskus, Ayse Gokce
buir.contributor.authorTombaz, Melike
buir.contributor.authorKonu, Ozlen
buir.contributor.orcidKeskus, Ayse Gokce|0000-0002-3934-8587
buir.contributor.orcidTombaz, Melike|0000-0002-0528-6680
buir.contributor.orcidKonu, Ozlen|0000-0002-6223-5329
dc.citation.epage124en_US
dc.citation.issueNumber1
dc.citation.spage111en_US
dc.citation.volumeNumber35en_US
dc.contributor.authorCagnan, I.
dc.contributor.authorKeles, M.
dc.contributor.authorKeskus, Ayse Gokce
dc.contributor.authorTombaz, Melike
dc.contributor.authorSahan, O. B.
dc.contributor.authorAerts-Kaya, F.
dc.contributor.authorUckan-Cetinkaya, D.
dc.contributor.authorKonu, Ozlen
dc.contributor.authorGunel-Ozcan, A.
dc.date.accessioned2022-02-17T08:53:04Z
dc.date.available2022-02-17T08:53:04Z
dc.date.issued2021-11-18
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.departmentInterdisciplinary Program in Neuroscience (NEUROSCIENCE)en_US
dc.description.abstractFanconi anemia (FA) is a rare genetic disorder characterized by genomic instability, developmental defects, and bone marrow (BM) failure. Hematopoietic stem cells (HSCs) in BM interact with the mesenchymal stem/stromal cells (MSCs); and this partly sustains the tissue homeostasis. MicroRNAs (miRNAs) can play a critical role during these interactions possibly via paracrine mechanisms. This is the first study addressing the miRNA profile of FA BM–MSCs obtained before and after BM transplantation (preBMT and postBMT, respectively). Non-coding RNA expression profiling and quality control analyses were performed in Donors (n = 13), FA preBMT (n = 11), and FA postBMT (n = 6) BM–MSCs using GeneChip miRNA 2.0 Array. Six Donor-FA preBMT pairs were used to identify a differentially expressed miRNA expression signature containing 50 miRNAs, which exhibited a strong correlation with the signature obtained from unpaired samples. Five miRNAs (hsa-miR-146a-5p, hsa-miR-148b-3p, hsa-miR-187-3p, hsa-miR-196b-5p, and hsa-miR-25-3p) significantly downregulated in both the paired and unpaired analyses were used to generate the BM–MSCs’ miRNA—BM mononuclear mRNA networks upon integration of a public dataset (GSE16334; studying Donor versus FA samples). Functionally enriched KEGG pathways included cellular senescence, miRNAs, and pathways in cancer. Here, we showed that hsa-miR-146a-5p and hsa-miR-874-3p were rescued upon BMT (n = 3 triplets). The decrease in miR-146a-5p was also validated using RT-qPCR and emerged as a strong candidate as a modulator of BM mRNAs in FA patients.en_US
dc.description.provenanceSubmitted by Dilan Ayverdi (dilan.ayverdi@bilkent.edu.tr) on 2022-02-17T08:53:03Z No. of bitstreams: 1 Global_miRNA_expression_of_bone_marrow_mesenchymal_stem_stromal_cells_derived_from_Fanconi_anemia_patients.pdf: 3110047 bytes, checksum: 0e2445c0533586c9a38fddfcfa2a6ec3 (MD5)en
dc.description.provenanceMade available in DSpace on 2022-02-17T08:53:04Z (GMT). No. of bitstreams: 1 Global_miRNA_expression_of_bone_marrow_mesenchymal_stem_stromal_cells_derived_from_Fanconi_anemia_patients.pdf: 3110047 bytes, checksum: 0e2445c0533586c9a38fddfcfa2a6ec3 (MD5) Previous issue date: 2022-01en
dc.identifier.doi10.1007/s13577-021-00626-9en_US
dc.identifier.eissn1749-0774
dc.identifier.issn0914-7470
dc.identifier.urihttp://hdl.handle.net/11693/77459
dc.language.isoEnglishen_US
dc.publisherSpringeren_US
dc.relation.isversionofhttps://doi.org/10.1007/s13577-021-00626-9en_US
dc.source.titleHuman Cellen_US
dc.subjectFanconi anemiaen_US
dc.subjectBone marrowen_US
dc.subjectMesenchymal stem/stromal cellsen_US
dc.subjectmiRNAen_US
dc.subjectNon-coding RNAsen_US
dc.titleGlobal miRNA expression of bone marrow mesenchymal stem/stromal cells derived from Fanconi anemia patientsen_US
dc.typeArticleen_US

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Department of Molecular Biology and Genetics
Aysel Sabuncu Brain Research Center (BAM)