Characterization of self-assembly and self-healing of peptide amphiphiles by atomic force microscopy

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buir.advisorGüler, Mustafa Özgür
dc.contributor.authorDikeçoğlu, Fatma Begüm
dc.date.accessioned2017-11-07T13:33:11Z
dc.date.available2017-11-07T13:33:11Z
dc.date.copyright2017-10
dc.date.issued2017-10
dc.date.submitted2017-11-07
dc.descriptionCataloged from PDF version of article.en_US
dc.descriptionThesis (M.S.): Bilkent University, Department of Materials Science and Nanotechnology, İhsan Doğramacı Bilkent University, 2017.en_US
dc.descriptionIncludes bibliographical references (leaves 58-63).en_US
dc.description.abstractBiological feedback mechanisms exert precise control over the initiation and termination of molecular self-assembly in response to environmental stimuli, while minimizing the formation and propagation of defects through self-repair processes. Peptide amphiphile (PA) molecules can self-assemble at physiological conditions to form supramolecular nanostructures that structurally and functionally resemble the nanofibrous proteins of the extracellular matrix (ECM), and their ability to reconfigure themselves in response to external stimuli is crucial for the design of intelligent systems. In this thesis, we investigated the real-time self-assembly, deformation, and self-healing of ECM-mimetic PA nanofibers in aqueous solution by using a force-stabilizing double-pass scanning AFM imaging method to disrupt the self-assembled peptide nanofibers in a force-dependent manner. We showed that nanofiber damage occurs at tip forces exceeding 1 nN, and that the damaged fibers subsequently recover under sub-nN tip forces. Fiber ends occasionally failed to reconnect following breakage and continue to grow as two individual nanofibers. Energy minimization calculations of nanofibers with increasing cross-sectional ellipticity (corresponding to varying levels of tip-induced fiber deformation) supported our observations, with high-ellipticity nanofibers exhibiting lower stability compared to their non-deformed counterparts. As a result, tip-mediated mechanical forces can provide an effective means of altering nanofiber integrity and visualizing the self-recovery of PA assemblies.en_US
dc.description.provenanceSubmitted by Betül Özen (ozen@bilkent.edu.tr) on 2017-11-07T13:33:11Z No. of bitstreams: 1 Tez_BegumDikecoglu_10169068.pdf: 4553190 bytes, checksum: 32931b52b53aa5aee766399835951c97 (MD5)en
dc.description.provenanceMade available in DSpace on 2017-11-07T13:33:11Z (GMT). No. of bitstreams: 1 Tez_BegumDikecoglu_10169068.pdf: 4553190 bytes, checksum: 32931b52b53aa5aee766399835951c97 (MD5) Previous issue date: 2017-11en
dc.description.statementofresponsibilityby Fatma Begüm Dikeçoğlu.en_US
dc.embargo.release2020-10-01
dc.format.extentxvi, 63 leaves : illustrations (some color), charts (some color) ; 30 cmen_US
dc.identifier.itemidB019952
dc.identifier.urihttp://hdl.handle.net/11693/33870
dc.language.isoEnglishen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSelf-assemblyen_US
dc.subjectSelf-healingen_US
dc.subjectPeptide amphiphile nanofibersen_US
dc.subjectAtomic force microscopyen_US
dc.titleCharacterization of self-assembly and self-healing of peptide amphiphiles by atomic force microscopyen_US
dc.title.alternativePeptit nanofiber yapılarının atomik kuvvet mikroskopu ile ıncelenmesien_US
dc.typeThesisen_US
thesis.degree.disciplineMaterials Science and Nanotechnology
thesis.degree.grantorBilkent University
thesis.degree.levelMaster's
thesis.degree.nameMS (Master of Science)

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