Identification of genes induced by BRCA1 in breast cancer cells

dc.citation.epage846en_US
dc.citation.issueNumber5en_US
dc.citation.spage839en_US
dc.citation.volumeNumber299en_US
dc.contributor.authorAtalay, A.en_US
dc.contributor.authorCrook, T.en_US
dc.contributor.authorOzturk, M.en_US
dc.contributor.authorYulug, I. G.en_US
dc.date.accessioned2016-02-08T10:31:52Z
dc.date.available2016-02-08T10:31:52Z
dc.date.issued2002en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractInherited mutations of the BRCA1 gene predispose to breast, ovarian, and other cancers. The role of the BRCA1 gene in the maintenance of chromosomal integrity is linked to a number of biological properties of its protein product, including transcriptional regulation. In the present study, we have used suppression subtractive hybridisation (SSH) to identify genes induced by BRCA1 by comparing control MCF7 breast carcinoma cells (driver) with MCF7 cells ectopically expressing BRCA1 (tester) and generated a forward subtracted cDNA library. We screened 500 putative positive clones from this library. Two hundred and ten of these clones were positive by differential screening with forward and reverse subtracted probes and the 65 cDNA clones which showed more than fivefold increase were selected for sequencing analysis. We clustered 46 different genes that share high homology with sequences in the GenBank/EMBL databases. Among these, 30 were genes whose function had been previously identified while the remaining 16 clones were genes with unknown functions. Of particular interest, BRCA1 gene induces the expression of genes encoding DNA repair proteins RAD21 and MSH2, ERBB2/HER2 interacting protein ERBIN, meningioma-associated protein MAC30, and a candidate ovarian tumour-suppressor OVCA1. Northern and Western blot analyses confirmed that the expression of these five genes are up-regulated following BRCA1 overexpression in MCF7 and UBR60-bcl2 cells. This is the first study reporting a set of BRCA1-induced genes in breast carcinoma cells by the SSH technique. We suggest that some known genes identified in this study may provide new insights into the tumour-suppressor function of BRCA1. © 2002 Elsevier Science (USA). All rights reserved.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:31:52Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2002en
dc.identifier.doi10.1016/S0006-291X(02)02751-1en_US
dc.identifier.issn0006-291X
dc.identifier.urihttp://hdl.handle.net/11693/24617
dc.language.isoEnglishen_US
dc.publisherAcademic pressen_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/S0006-291X(02)02751-1en_US
dc.source.titleBiochemical and Biophysical Research Communicationsen_US
dc.subjectBRCA1en_US
dc.subjectBreast canceren_US
dc.subjectGene expressionen_US
dc.subjectMCF7en_US
dc.subjectSSHen_US
dc.subjectTarget geneen_US
dc.titleIdentification of genes induced by BRCA1 in breast cancer cellsen_US
dc.typeArticleen_US

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