Frequent demonstration of human herpesvirus 8 (HHV-8) in bone marrow biopsy samples from Turkish patients with multiple myeloma (MM)

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dc.citation.epage1273en_US
dc.citation.issueNumber8en_US
dc.citation.spage1268en_US
dc.citation.volumeNumber15en_US
dc.contributor.authorBeksac, M.en_US
dc.contributor.authorMa, M.en_US
dc.contributor.authorAkyerli, C.en_US
dc.contributor.authorDerDanielian, M.en_US
dc.contributor.authorZhang, L.en_US
dc.contributor.authorLiu, J.en_US
dc.contributor.authorArat, M.en_US
dc.contributor.authorKonuk, N.en_US
dc.contributor.authorKoc, H.en_US
dc.contributor.authorOzcelik, T.en_US
dc.contributor.authorVescio, R.en_US
dc.contributor.authorBerenson, J. R.en_US
dc.date.accessioned2016-02-08T10:34:44Z
dc.date.available2016-02-08T10:34:44Z
dc.date.issued2001en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractIn order to investigate the frequency of HHV-8 in MM patients from another geographic location, we obtained fresh bone marrow (BM) biopsies from Turkish patients with MM (n = 21), monoclonal gammopathy of undetermined significance (MGUS) (n = 2), plasmacytoma (n = 1) with BM plasma cell infiltration, various hematological disorders (n = 6), and five healthy Turkish controls. The frequency of HHV-8 was analyzed by polymerase chain reaction (PCR) in two independent laboratories in the USA and in Turkey. Using fresh BM biopsies, 17/21 MM patients were positive for HHV-8 whereas all five healthy controls, and six patients with other hematological disorders were negative. Two patients with MGUS, and one patient with a solitary plasmacytoma were also negative. The data from the two laboratories were completely concordant. Also using primer pairs for v IRF and v IL-8R confirmed the results observed with the KS330233 primers. Furthermore, sequence analysis demonstrated a C3 strain pattern in the ORF26 region which was also found in MM patients from the US. Thus, HHV-8 is present in the majority of Turkish MM patients, and the absence of the virus in healthy controls further supports its role in the pathogenesis of MM.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:34:44Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2001en
dc.identifier.doi10.1038/sj.leu.2402190en_US
dc.identifier.issn0887-6924
dc.identifier.urihttp://hdl.handle.net/11693/24811
dc.language.isoEnglishen_US
dc.publisherNature Publishingen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/sj.leu.2402190en_US
dc.source.titleLeukemiaen_US
dc.subjectFrequency and sequenceen_US
dc.subjectHHV-8en_US
dc.subjectMultiple myelomaen_US
dc.subjectTurkish patientsen_US
dc.subjectImmunoglobulin heavy chainen_US
dc.subjectImmunoglobulin receptoren_US
dc.subjectInterleukin 8 receptoren_US
dc.subjectPlasma proteinen_US
dc.subjectPrimer DNAen_US
dc.subjectBasement membraneen_US
dc.subjectBone marrow biopsyen_US
dc.subjectCell infiltrationen_US
dc.subjectClinical articleen_US
dc.subjectControlled studyen_US
dc.subjectFrequency analysisen_US
dc.subjectGeographic distributionen_US
dc.subjectHematologic diseaseen_US
dc.subjectHuman herpesvirus 8en_US
dc.subjectHuman tissueen_US
dc.subjectLaboratory testen_US
dc.subjectMonoclonal immunoglobulinemiaen_US
dc.subjectMultiple myelomaen_US
dc.subjectNonhumanen_US
dc.subjectOpen reading frameen_US
dc.subjectPathogenesisen_US
dc.subjectPlasma cellen_US
dc.subjectPlasmacytomaen_US
dc.subjectPolymerase chain reactionen_US
dc.subjectPriority journalen_US
dc.subjectSequence analysisen_US
dc.subjectTurkey (republic)en_US
dc.subjectUnited Statesen_US
dc.subjectVirus detectionen_US
dc.subjectVirus strainen_US
dc.subjectBiopsyen_US
dc.subjectBone Marrowen_US
dc.subjectHerpesviridae infectionsen_US
dc.subjectHerpesvirus 8, humanen_US
dc.subjectHumansen_US
dc.subjectMultiple Myelomaen_US
dc.subjectTurkeyen_US
dc.titleFrequent demonstration of human herpesvirus 8 (HHV-8) in bone marrow biopsy samples from Turkish patients with multiple myeloma (MM)en_US
dc.typeArticleen_US

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