Expression of key synaptic proteins in Zebrafish (Danio Rerio) brain following caloric restriction and its mimetic and their relationship with gender

dc.contributor.advisorAdams, Michelle Marie
dc.contributor.authorDede, Ayşegül
dc.departmentGraduate Program in Neuroscienceen_US
dc.descriptionCataloged from PDF version of article.en_US
dc.descriptionThesis (M.S.): Bilkent University, Department of Neuroscience, İhsan Doğramacı Bilkent University, 2017.en_US
dc.descriptionIncludes bibliographical references (leaves 86-114).en_US
dc.description.abstractAging is a progressive decline of physiological functioning and metabolic processes. Among all the organs, the brain seems to be the most vulnerable part of the body to the age-related changes because of the relatively high consumption of oxygen and glucose as compared to other organs. Both structural and cognitive changes occur during the aging process. A great effort has been spent to ameliorate the outcomes occurring within the brain as a result of aging. Caloric restriction (CR) is considered to be the only non-genetic intervention which decreases age-related cognitive decline. Rapamycin (RAP) has become a candidate drug which was shown to mimic the effects of CR by blocking the nutrient-sensing pathway, the mammalian target of Rapamycin, (mTOR) pathway. The first aim of this study was to investigate the expressions of key synaptic proteins; gephyrin, PSD-95 and synaptophysin, which are involved in the synaptic plasticity, after short-term (4 weeks) CR and RAP interventions in young and old, male and female zebrafish. The second aim was to investigate whether the expression of glutamate receptor subunits, NR2B and GluR2/3, display a sexually dimorphic pattern in middle age zebrafish. It was found that there was no significant difference in the expression of key synaptic proteins between the CR and RAP animal groups as compared to the ad libitium (AL) fed group and also no significance was found in the expression of NR2B and GluR2/3 in middle-aged male and female zebrafish. Highlighted studies in this thesis demonstrate that short-term (4 weeks) of CR and RAP treatments were too short to observe an effect in the expression level of gephyrin, synaptophysin, and PSD-95, and in the middle age, expression of NR2B and GluR2/3 did not display sexually dimorphic pattern. Our initial results of key synaptic protein levels indicate that they are stable throughout aging with respect to gender and CR interventions.en_US
dc.description.statementofresponsibilityby Ayşegül Dede.en_US
dc.format.extentxvii, 114 leaves : charts.en_US
dc.publisherBilkent Universityen_US
dc.subjectBrain agingen_US
dc.subjectCaloric restrictionen_US
dc.subjectSexual dimorphismen_US
dc.subjectSynaptic proteinsen_US
dc.subjectGlutamate receptor subunitsen_US
dc.titleExpression of key synaptic proteins in Zebrafish (Danio Rerio) brain following caloric restriction and its mimetic and their relationship with genderen_US
dc.title.alternativeZebra balığı (Danio Rerio) beynindeki anahtar sinaptik protein ifadelerinin kısa sureli kalori kısıtlaması ve kalori kısıtlaması taklitçisi ve cinsiyetle ilişkisien_US
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