Molecular cloning and characterization of the common 1b subtype of HCV from Turkey

Simple item page
buir.advisorÖztürk, Mehmet
dc.contributor.authorÖztan, Aslı
dc.date.accessioned2016-01-08T20:16:32Z
dc.date.available2016-01-08T20:16:32Z
dc.date.issued1999
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.descriptionAnkara : Department of Molecular Biology and Genetics and Institute of Engineering and Science, Bilkent University, 1999.en_US
dc.descriptionThesis (Master's) -- Bilkent University, 1999.en_US
dc.descriptionIncludes bibliographical references leaves 64-71.en_US
dc.description.abstractHepatitis C Virus is a major cause of acute and chronic hepatitis worldwide. 80-90% of Hepatitis C Virus infections become chronic and 75% of these cases lead to liver disease, including cirrhosis, liver failure and hepatocellular carcinoma. Hepatitis C Virus was first identified by molecular cloning of the viral genome in 1989. Hepatitis C Virus is an enveloped virus containing a positive stranded RNA genome with a size of around 9.5 kilobases. In terms of genomic organization, it was accepted as a member of Flaviviridae family as a new genus named Hepaciviruses. The single-stranded RNA genome encodes a single open reading frame, which is transcribed into a single polypeptide of 3010 or 3030 amino acids and cleaved into viral proteins Core, E l, E2/p7, NS2, NS3, NS4A, NS4B, NS5A, NS5B by host and viral proteases. Sequencing, serotyping and RFLP studies indicate that Hepatitis C Virus genome is highly variable. There are six distinct genotypes and at least 74 subtypes with different distributions between the geographic areas. Variability is not equally distributed throughout the genome. 5' UTR, some parts of the 3' UTR and capsid protein are the most conserved regions. The predominant genotype in the Turkish population was found to be lb by sequencing of the 5-UTR. In this study, the entire sequence encompassing the complete coding region and partial non coding regions of the genotype lb obtained from a HCV-infected Turkish patient was cloned to investigate its evolutionary relationship with other genotypes and to study its overall genome organization,. In order to characterize the viral genome, viral RNA was extracted from the serum, cDNA was synthesized, the HCV genome was amplified by PCR in 7 overlapping fragments, PCR fragments were cloned into bacterial vectors and cloned inserts were sequenced by automated sequencing methodology. The partial sequence data covering 70% of the cloned HCV genome indicate that the Turkish lb genotype displays high homology to other lb genotypes, but differs from others by distinct amino acid changes. To our knowledge, this is the first report about the HCV genome structure from Turkey. The HCV subgenomic fragments obtained here will serve to further molecular and immunologic studies on this dominant form of HCV found in Turkish patients.en_US
dc.description.degreeM.S.en_US
dc.description.provenanceMade available in DSpace on 2016-01-08T20:16:32Z (GMT). No. of bitstreams: 1 1.pdf: 78510 bytes, checksum: d85492f20c2362aa2bcf4aad49380397 (MD5)en
dc.description.statementofresponsibilityÖztan, Aslıen_US
dc.format.extentxi, 75 leavesen_US
dc.identifier.urihttp://hdl.handle.net/11693/18132
dc.language.isoEnglishen_US
dc.publisherBilkent Universityen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHepatitis C Virusen_US
dc.subjectlben_US
dc.subjectgenomeen_US
dc.subjectcloningen_US
dc.subjectsequencingen_US
dc.subjectTurkish Isolateen_US
dc.subject.lccQR201.H46 O98 1999en_US
dc.subject.lcshHepatitis C virus..en_US
dc.titleMolecular cloning and characterization of the common 1b subtype of HCV from Turkeyen_US
dc.typeThesisen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
B049052.pdf
Size:
7.62 MB
Format:
Adobe Portable Document Format
Description:
Full printable version
Download

Collections

Dept. of Molecular Biology and Genetics - Master's degree