Differential expression of colon cancer associated transcript1 (CCAT1) along the colonic adenoma-carcinoma sequence

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dc.citation.volumeNumber13en_US
dc.contributor.authorAlaiyan, B.en_US
dc.contributor.authorIlyayev, N.en_US
dc.contributor.authorStojadinovic, A.en_US
dc.contributor.authorIzadjoo, M.en_US
dc.contributor.authorRoistacher, M.en_US
dc.contributor.authorPavlov, V.en_US
dc.contributor.authorTzivin, V.en_US
dc.contributor.authorHalle, D.en_US
dc.contributor.authorPan, H.en_US
dc.contributor.authorTrink, B.en_US
dc.contributor.authorGure, A. O.en_US
dc.contributor.authorNissan, A.en_US
dc.date.accessioned2016-02-08T09:39:26Z
dc.date.available2016-02-08T09:39:26Z
dc.date.issued2013en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractBackground: The transition from normal epithelium to adenoma and, to invasive carcinoma in the human colon is associated with acquired molecular events taking 5-10 years for malignant transformation. We discovered CCAT1, a non-coding RNA over-expressed in colon cancer (CC), but not in normal tissues, thereby making it a potential disease-specific biomarker. We aimed to define and validate CCAT1 as a CC-specific biomarker, and to study CCAT1 expression across the adenoma-carcinoma sequence of CC tumorigenesis.Methods: Tissue samples were obtained from patients undergoing resection for colonic adenoma(s) or carcinoma. Normal colonic tissue (n = 10), adenomatous polyps (n = 18), primary tumor tissue (n = 22), normal mucosa adjacent to primary tumor (n = 16), and lymph node(s) (n = 20), liver (n = 8), and peritoneal metastases (n = 19) were studied. RNA was extracted from all tissue samples, and CCAT1 expression was analyzed using quantitative real time-PCR (qRT-PCR) with confirmatory in-situ hybridization (ISH).Results: Borderline expression of CCAT1 was identified in normal tissue obtained from patients with benign conditions [mean Relative Quantity (RQ) = 5.9]. Significant relative CCAT1 up-regulation was observed in adenomatous polyps (RQ = 178.6 ± 157.0; p = 0.0012); primary tumor tissue (RQ = 64.9 ± 56.9; p = 0.0048); normal mucosa adjacent to primary tumor (RQ = 17.7 ± 21.5; p = 0.09); lymph node, liver and peritoneal metastases (RQ = 11,414.5 ± 12,672.9; 119.2 ± 138.9; 816.3 ± 2,736.1; p = 0.0001, respectively). qRT-PCR results were confirmed by ISH, demonstrating significant correlation between CCAT1 up-regulation measured using these two methods.Conclusion: CCAT1 is up-regulated across the colon adenoma-carcinoma sequence. This up-regulation is evident in pre-malignant conditions and through all disease stages, including advanced metastatic disease suggesting a role in both tumorigenesis and the metastatic process. © 2013 Alaiyan et al.; licensee BioMed Central Ltd.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T09:39:26Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2013en
dc.identifier.doi10.1186/1471-2407-13-196en_US
dc.identifier.issn1471-2407
dc.identifier.urihttp://hdl.handle.net/11693/21005
dc.language.isoEnglishen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofhttp://dx.doi.org/10.1186/1471-2407-13-196en_US
dc.source.titleBMC Canceren_US
dc.subjectColon canceren_US
dc.subjectNon-coding RNAen_US
dc.subjectBiomarkersen_US
dc.subjectAdenomaen_US
dc.subjectCarcinomaen_US
dc.titleDifferential expression of colon cancer associated transcript1 (CCAT1) along the colonic adenoma-carcinoma sequenceen_US
dc.typeArticleen_US

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