An investigation of microRNAs mapping to breast cancer related genomic gain and loss regions

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dc.citation.epage23en_US
dc.citation.issueNumber1en_US
dc.citation.spage15en_US
dc.citation.volumeNumber189en_US
dc.contributor.authorSelcuklu, S. D.en_US
dc.contributor.authorYakicier, M. C.en_US
dc.contributor.authorErson, A. E.en_US
dc.date.accessioned2016-02-08T10:05:26Z
dc.date.available2016-02-08T10:05:26Z
dc.date.issued2009en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractVarious regions of amplification or loss are observed in breast tumors as a manifestation of genomic instability. To date, numerous oncogenes or tumor suppressors on some of these regions have been characterized. An increasing body of evidence suggests that such regions also harbor microRNA genes with crucial regulatory roles in cellular processes and disease mechanisms, including cancer. Here, we investigated 35 microRNAs localized to common genomic gain and/or loss regions in breast cancers. To examine amplification or loss of these microRNAs as a result of genomic instability, we performed semiquantitative duplex polymerase chain reaction in 20 breast cancer cell lines, 2 immortalized mammary cell lines, and 2 normal DNA controls. A comprehensive DNA fold number change data for 35 microRNA genes on chromosomal gain/loss regions are presented in breast cancer cells. A 23% (8/35) of the investigated microRNAs showed significant fold number increases (greater than fourfold) compared to GAPDH in one or more of the breast cell lines. Although no homozygous deletions were detected, fold number decreases indicating potential loss regions were observed for 26% (9/35) of the investigated microRNAs. Such fold number changes may point out some of these microRNAs as potential targets of the genomic instability regions as oncogene and tumor suppressor candidates. © 2009 Elsevier Inc. All rights reserved.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:05:26Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2009en
dc.identifier.doi10.1016/j.cancergencyto.2008.09.009en_US
dc.identifier.issn0165-4608
dc.identifier.urihttp://hdl.handle.net/11693/22841
dc.language.isoEnglishen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.cancergencyto.2008.09.009en_US
dc.source.titleCancer Genetics and Cytogeneticsen_US
dc.subjectMicroRNAen_US
dc.subjectBreast canceren_US
dc.subjectChromosome deletionen_US
dc.subjectChromosome lossen_US
dc.subjectControlled studyen_US
dc.subjectGenomic instabilityen_US
dc.subjectHumanen_US
dc.subjectHuman cellen_US
dc.subjectPriority journalen_US
dc.subjectReverse transcription polymerase chain reactionen_US
dc.titleAn investigation of microRNAs mapping to breast cancer related genomic gain and loss regionsen_US
dc.typeArticleen_US

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