Timing of induction of cardiomyocyte differentiation for in vitro cultured mesenchymal stem cells: a perspective for emergencies

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dc.citation.epage150en_US
dc.citation.issueNumber2en_US
dc.citation.spage143en_US
dc.citation.volumeNumber87en_US
dc.contributor.authorTokçaer-Keskin, Zeynepen_US
dc.contributor.authorAkar, A. R.en_US
dc.contributor.authorAyaloğlu-Bütün, Fatmaen_US
dc.contributor.authorTerzioğlu-Kara, Eceen_US
dc.contributor.authorDurdu, S.en_US
dc.contributor.authorÖzyurda, U.en_US
dc.contributor.authorUğur, M.en_US
dc.contributor.authorAkçalı, Kamil C.en_US
dc.coverage.spatialİstanbul, Turkeyen_US
dc.date.accessioned2016-02-08T11:34:46Zen_US
dc.date.available2016-02-08T11:34:46Zen_US
dc.date.issued2009en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.descriptionDate of Conference: 12-16 May 2008en_US
dc.descriptionConference Name: NATO Advanced Research Workshop on Translational Knowledge for Heart Health, 2008en_US
dc.description.abstractMesenchymal stem cells (MSCs) have the capacity to differentiate into osteoblasts, chondrocytes, adipocytes, myocytes, and cardiomyocytes. Several established methods are presently available for in vitro isolation of MSCs from bone marrow. However, the duration necessary to culture them can be a major handicap to cell-based therapies needed for such urgent cardiovascular conditions as acute myocardial infarction and acute hindlimb ischemia. The best timing of car- diomyocyte differentiation induction after MCS isolation and expansion is still an unresolved issue. Our goal was to investigate the possibility of obtaining functional cardiomyocytes from rat MSC within a shorter time period. We examined MSCs' colony-forming capacity, CD90 and CD34 immunoreactivity during the 14 days of culturing. Cardiomyocyte differentiation was induced by 5-azacytidine. Immunohistochemic staining, together with intracellular Ca2+ measurement experiments, revealed that MSCs do not differentiate into any specific cell lineage but show the characteristics of MSCs on both the 9th and 14th days of the culture. To check the potential for differentiation into cardiomyocytes, experiments with caffeine application and depolarization with KCl were performed. The cells possessed some of the specific biochemical features of contracting cells, with slightly higher capacities on the 14th day. Cells from 9th and 14th days of the culture that were treated with 5-azacytidine had a higher expression of cardiac-specific markers such as troponin I, α-sarcomeric actin, and MEF2D compared with the control groups. This study illustrates that it is possible to get functional cardiomyocytes from in vitro MSC culture in a shorter time period than previously achieved. This reduction in time may provide emergency cases with access to cell-based therapies that may have previously been unavailable.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T11:34:46Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2009en
dc.identifier.doi10.1139/Y08-111en_US
dc.identifier.issn0008-4212en_US
dc.identifier.urihttp://hdl.handle.net/11693/26752en_US
dc.language.isoEnglishen_US
dc.publisherNRC Research Pressen_US
dc.relation.isversionofhttp://dx.doi.org/10.1139/Y08-111en_US
dc.source.titleCanadian Journal of Physiology and Pharmacologyen_US
dc.subjectCardiomyocytesen_US
dc.subjectDifferentiationen_US
dc.subjectGene expressionen_US
dc.subjectIn vitroen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectRaten_US
dc.subjectAlpha actinen_US
dc.subjectAzacitidineen_US
dc.subjectCalciumen_US
dc.subjectGlyceraldehyde 3 phosphate dehydrogenaseen_US
dc.subjectMyocyte enhancer factor 2en_US
dc.subjectTroponin Ien_US
dc.subjectUnclassified drugen_US
dc.titleTiming of induction of cardiomyocyte differentiation for in vitro cultured mesenchymal stem cells: a perspective for emergenciesen_US
dc.typeConference Paperen_US

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