Histone H3.3 regulates mitotic progression in mouse embryonic fibroblasts

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dc.citation.epage499en_US
dc.citation.issueNumber4en_US
dc.citation.spage491en_US
dc.citation.volumeNumber95en_US
dc.contributor.authorOrs, A.en_US
dc.contributor.authorPapin, C.en_US
dc.contributor.authorFavier, B.en_US
dc.contributor.authorRoulland, Y.en_US
dc.contributor.authorDalkara, D.en_US
dc.contributor.authorOzturk, M.en_US
dc.contributor.authorHamiche, A.en_US
dc.contributor.authorDimitrov, S.en_US
dc.contributor.authorPadmanabhan, K.en_US
dc.date.accessioned2018-04-12T11:01:57Z
dc.date.available2018-04-12T11:01:57Z
dc.date.issued2017en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractH3.3 is a histone variant that marks transcription start sites as well as telomeres and heterochromatic sites on the genome. The presence of H3.3 is thought to positively correlate with the transcriptional status of its target genes. Using a conditional genetic strategy against H3.3B, combined with short hairpin RNAs against H3.3A, we essentially depleted all H3.3 gene expression in mouse embryonic fibroblasts. Following nearly complete loss of H3.3 in the cells, our transcriptomic analyses show very little impact on global gene expression or on the localization of histone variant H2A.Z. Instead, fibroblasts displayed slower cell growth and an increase in cell death, coincident with large-scale chromosome misalignment in mitosis and large polylobed or micronuclei in interphase cells. Thus, we conclude that H3.3 may have an important under-explored additional role in chromosome segregation, nuclear structure, and the maintenance of genome integrity. © 2017 Published by NRC Research Press.en_US
dc.description.provenanceMade available in DSpace on 2018-04-12T11:01:57Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 179475 bytes, checksum: ea0bedeb05ac9ccfb983c327e155f0c2 (MD5) Previous issue date: 2017en
dc.identifier.doi10.1139/bcb-2016-0190en_US
dc.identifier.issn0829-8211
dc.identifier.urihttp://hdl.handle.net/11693/37073
dc.language.isoEnglishen_US
dc.publisherCanadian Science Publishingen_US
dc.relation.isversionofhttp://dx.doi.org/10.1139/bcb-2016-0190en_US
dc.source.titleBiochemistry and Cell Biologyen_US
dc.subjectH3.3en_US
dc.subjectMitosisen_US
dc.subjectMouse embryonic fibroblastsen_US
dc.subjectRNA-seqen_US
dc.subjectTranscriptionen_US
dc.titleHistone H3.3 regulates mitotic progression in mouse embryonic fibroblastsen_US
dc.typeArticleen_US

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